大鼠颞下颌关节酵母聚糖诱导性关节炎的实验模型：一氧化氮和中性粒细胞的作用

Experimental model of zymosan-induced arthritis in the rat temporomandibular joint: role of nitric oxide and neutrophils.

作者信息

Chaves Hellíada Vasconcelos, Ribeiro Ronaldo de Albuquerque, de Souza André Mattos Brito, Rodrigues e Silva Antonio Alfredo, Gomes Antoniella Souza, Vale Mariana Lima, Bezerra Mirna Marques, Brito Gerly Anne de Castro

机构信息

Faculty of Dentistry, Federal University of Ceará, Sobral Campus, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, 62042-280 Sobral, CE, Brazil.

出版信息

J Biomed Biotechnol. 2011;2011:707985. doi: 10.1155/2011/707985. Epub 2011 Jan 3.

AIMS

To establish a new model of zymosan-induced temporomandibular joint (TMJ) arthritis in the rat and to investigate the role of nitric oxide.

METHODS

Inflammation was induced by an intra-articular injection of zymosan into the left TMJ. Mechanical hypernociception, cell influx, vascular permeability, myeloperoxidase activity, nitrite levels, and histological changes were measured in TMJ lavages or tissues at selected time points. These parameters were also evaluated after treatment with the nitric oxide synthase (NOS) inhibitors L-NAME or 1400 W.

RESULTS

Zymosan-induced TMJ arthritis caused a time-dependent leucocyte migration, plasma extravasation, mechanical hypernociception, and neutrophil accumulation between 4 and 24 h. TMJ immunohistochemical analyses showed increased inducible NOS expression. Treatment with L-NAME or 1400 W inhibited these parameters.

CONCLUSION

Zymosan-induced TMJ arthritis is a reproducible model that may be used to assess both the mechanisms underlying TMJ inflammation and the potential tools for therapies. Nitric oxide may participate in the inflammatory temporomandibular dysfunction mechanisms.