Postgraduate program in Pharmaceutical Science / Department of Biophysics and Pharmacology, UFRN, Natal, RN, Brazil.
Department of Biophysics and Pharmacology, UFRN, Natal, RN, Brazil.
BMC Complement Altern Med. 2019 Feb 12;19(1):47. doi: 10.1186/s12906-019-2454-3.
Libidibia ferrea (L. ferrea) has been used in folk medicine to treat several conditions and to prevent cancer. This study performed a chromatographic analysis of the crude aqueous extract of Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz (LfAE) leaves and evaluated its in vivo antioxidant and anti-inflammatory potential.
Polyphenols present in LfAE were characterized by high performance liquid chromatography (HPLC). Anti-inflammatory activity was studied in an experimental model of zymosan-induced intra-articular inflammation, conducted in Wistar rats treated with LfAE at the doses of 100, 200 and 300 mg/kg by gavage. Synovial fluid was collected for global leukocyte count, for spectrocopical UV/VIS analysis of myeloperoxidase (MPO) activity, total glutathione and malondialdehyde (MDA), and for quantification of inflammatory cytokines IL1-β and TNF-α by enzyme-linked immunosorbent assay. Synovial membrane was collected for histological analysis. The level of statistical significance was p < 0.05.
HPLC detected concentrations of 1.56 (0.77) %m/m for ellagic acid and 1.20 (1.38) %m/m for gallic acid in LfAE leaves. Treatment with LfAE at all doses significantly decreased the leukocyte influx into the synovial fluid (p < 0.001) and myeloperoxidase activity (p < 0.001), an important marker of neutrophils. LfAE at doses of 100 (p < 0.05), 200 and 300 mg/kg (p < 0.001) also reduced the levels of MDA. LfAE at doses of 200 and 300 mg/kg significantly decreased the levels of IL-1β (p < 0.05) and TNF-α (p < 0.001). All doses of LfAE resulted in increased levels of total glutathione (p < 0.001). Histopathological findings confirmed a reduction of the inflammatory infiltrate in the rats treated with LfAE at a dose of 200 mg/kg (p < 0.05).
LfAE has an important anti-oxidant and anti-inflammatory effect on intra-articular inflammation.
利比迪比亚 ferrea（L. ferrea）在民间医学中被用于治疗多种疾病和预防癌症。本研究对利比迪比亚 ferrea（Mart. ex. Tul。）L.P. Queiroz 的粗水提物（LfAE）叶片进行了色谱分析，并评估了其体内抗氧化和抗炎潜力。
通过高效液相色谱法（HPLC）对 LfAE 中的多酚进行了表征。在佐剂诱导的关节内炎症实验模型中研究了抗炎活性，在 Wistar 大鼠中进行，LfAE 以 100、200 和 300mg/kg 的剂量经口灌胃。收集滑液进行总白细胞计数，分光光度法 UV/VIS 分析髓过氧化物酶（MPO）活性、总谷胱甘肽和丙二醛（MDA），以及通过酶联免疫吸附试验定量分析白细胞介素 1-β和肿瘤坏死因子-α（TNF-α）。收集滑膜膜进行组织学分析。统计显著性水平为 p<0.05。
HPLC 检测到 LfAE 叶片中鞣花酸的浓度为 1.56（0.77）%m/m，没食子酸的浓度为 1.20（1.38）%m/m。LfAE 以所有剂量治疗均显著降低白细胞流入滑液（p<0.001）和髓过氧化物酶活性（p<0.001），这是中性粒细胞的重要标志物。LfAE 以 100mg/kg（p<0.05）、200 和 300mg/kg（p<0.001）剂量还降低了 MDA 水平。LfAE 以 200 和 300mg/kg 剂量显著降低了 IL-1β（p<0.05）和 TNF-α（p<0.001）的水平。LfAE 的所有剂量均导致总谷胱甘肽水平升高（p<0.001）。组织病理学发现证实，在 200mg/kg LfAE 治疗的大鼠中炎症浸润减少（p<0.05）。
LfAE 对关节内炎症具有重要的抗氧化和抗炎作用。
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