Institute of Medicine, Chung Shan Medical University, No. 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:507920. doi: 10.1155/2011/507920. Epub 2011 Jan 10.
Recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of Dioscorea nipponica Makino whereas the effect of this plant on metastasis of cancer cells has not been clearly clarified. In the present study, we extracted Dioscorea nipponica Makino with methanol (DNE1), chloroform (DNE2), ethyl acetate (DNE3), n-butanol (DNE4), and water (DNE5). We first demonstrate that DNE3 was found to be effective in reducing the lung metastases formation by about 99.5% as compared to vehicle-treated control animals. When a nontoxic concentration of the extract was treated directly to highly metastatic murin melanoma cells (B16F10) and human melanoma cells (A2058) in vitro, it exerted a dose-dependent inhibitory effect on the invasion (P < .001), motility (P < .001), secretion of MMPs (P < .001), and u-PA (P < .001) of both cell lines. To investigate the possible mechanisms involved in these events, we performed western blot analysis to find that DNE inhibited phosphorylation of Akt. A treatment with DNE3 to B16F10 cells also inhibited the activation of NF-κB and increased the expression of IkappaB. Taken together, these findings suggested that DNE3 could reduce the metastasis of melanoma cells, thereby constituting an adjuvant treatment for metastasis control.
最近的研究揭示了穿龙薯蓣具有多种抗癌和抗增殖能力，而这种植物对癌细胞转移的影响尚未明确阐明。在本研究中，我们用甲醇（DNE1）、氯仿（DNE2）、乙酸乙酯（DNE3）、正丁醇（DNE4）和水（DNE5）提取了穿龙薯蓣。我们首先证明 DNE3 可有效减少约 99.5%的肺转移形成，与用对照溶剂处理的动物相比。当将非毒性浓度的提取物直接处理于体外高转移性鼠黑色素瘤细胞（B16F10）和人黑色素瘤细胞（A2058）时，它对侵袭（P <.001）、迁移（P <.001）、MMPs 分泌（P <.001）和 u-PA（P <.001）均表现出剂量依赖性抑制作用。为了研究这些事件中可能涉及的机制，我们进行了 Western blot 分析，发现 DNE 抑制 Akt 的磷酸化。用 DNE3 处理 B16F10 细胞也抑制了 NF-κB 的激活并增加了 IkappaB 的表达。综上所述，这些发现表明 DNE3 可减少黑色素瘤细胞的转移，从而构成转移控制的辅助治疗。
