Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
Mol Cell Biochem. 2011 Jun;352(1-2):19-24. doi: 10.1007/s11010-011-0735-3. Epub 2011 Jan 28.
Several studies have investigated that linoleic acid (LA) and eicosapentaenoic acid (EPA) affect cell proliferation and lipid catabolic gene expression in mammals. To determine if LA and EPA increase duck cell proliferation and lipid catabolic gene expression, the authors exposed duck primary hepatocyte cultures to LA or EPA. The results showed that both LA and EPA increased cell proliferation in a dose-dependent manner (100 μM). The effect on specific cell-cycle phases was also studied; LA and EPA (100 μM) deceased the proportion of cells in the G0/G1 phase from 83 to 80.8 and 80.3%, respectively, concomitant with an increase in the proportion of S-phase cells (11.5 and 10.5 vs. 8%, respectively). The expression of PPAR-α and PPAR-α target genes, such as acyl-CoA oxidase (ACOX), lipoprotein lipase (LPL), liver fatty acid-binding protein (L-FABP), was examined by quantitative real-time PCR. The results showed that the expression of the PPAR-α, ACOX, and LPL genes increased significantly following LA and EPA exposure, but that the expression of L-FABP remained unchanged. This study provides the first characterization of LA- and EPA-induced cell proliferation and PPAR-α and PPAR-α target gene transcriptional responses in duck primary hepatocyte cultures.
多项研究已经证实亚油酸（LA）和二十碳五烯酸（EPA）会影响哺乳动物的细胞增殖和脂质分解代谢基因表达。为了确定 LA 和 EPA 是否会增加鸭原代肝细胞的增殖和脂质分解代谢基因表达，作者将 LA 或 EPA 暴露于鸭原代肝细胞培养物中。结果表明，LA 和 EPA 均以剂量依赖的方式（100 μM）促进细胞增殖。作者还研究了它们对特定细胞周期阶段的影响；LA 和 EPA（100 μM）分别使 G0/G1 期细胞比例从 83%降低至 80.8%和 80.3%，同时 S 期细胞比例增加（分别为 11.5%和 10.5%，而 8%）。通过定量实时 PCR 检测了 PPAR-α 和 PPAR-α 靶基因（如酰基辅酶 A 氧化酶（ACOX）、脂蛋白脂肪酶（LPL）、肝脂肪酸结合蛋白（L-FABP）的表达。结果表明，LA 和 EPA 暴露后，PPAR-α、ACOX 和 LPL 基因的表达显著增加，但 L-FABP 的表达保持不变。本研究首次描述了 LA 和 EPA 诱导的鸭原代肝细胞增殖以及 PPAR-α 和 PPAR-α 靶基因转录反应。
