Lin Jia, Yi Xiaoping, Zhuang Yingping
State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology 130 Mei Long Road, Xu Hui District Shanghai 200237 China
RSC Adv. 2019 Aug 30;9(47):27369-27377. doi: 10.1039/c9ra05128g. eCollection 2019 Aug 29.
Chicken embryo fibroblast DF-1 cells are increasingly being used in the production of avian virus vaccines. However, the relatively low proliferative capacity does not meet the requirements of industrial production. In this study, we attempted to improve the proliferative capacity of DF-1 cells. The results of intracellular metabolomics showed that 28 types of metabolites could play roles in DF-1 cell growth based on the variance and timing analysis of intracellular metabolites from DF-1 cells grown in two media with distinct growth difference, DMEM/F12 (1 : 1) and DMEM. By examining the differences in the components in the two media, DOE was used to screen and optimize the growth medium for DF-1 cells. The maximum cell density was 40.72% higher, and the infectious bursal disease virus (IBDV) titer was 2.68 times higher, in the optimized medium than in the control. This study proposes a complete solution from metabolomics to media optimization.
鸡胚成纤维细胞DF-1越来越多地用于禽病毒疫苗的生产。然而，其相对较低的增殖能力不符合工业生产的要求。在本研究中，我们试图提高DF-1细胞的增殖能力。细胞内代谢组学结果表明，基于在具有明显生长差异的两种培养基（DMEM/F12(1:1)和DMEM）中生长的DF-1细胞内代谢物的方差和时间分析，28种代谢物可能在DF-1细胞生长中发挥作用。通过检查两种培养基中成分的差异，采用实验设计来筛选和优化DF-1细胞的生长培养基。与对照相比，优化后的培养基中最大细胞密度提高了40.72%，传染性法氏囊病病毒（IBDV）滴度提高了2.68倍。本研究提出了从代谢组学到培养基优化的完整解决方案。
