College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
J Fluoresc. 2011 Jul;21(4):1607-15. doi: 10.1007/s10895-011-0849-8. Epub 2011 Jan 28.
In the paper, 2,4,5-tri(2-furyl)-1H-imidazole (TFI) and 2-phenyl-4,5-di(2-furyl)-1H-imidazole (PDFI), were chosen to investigate chemiluminescence (CL) properties of 2-substituted-4,5-di(2-furyl)-1H-imidazoles. The directly oxidized CL of analytes by potassium permanganate (KMnO(4)) was in detail studied. The KMnO(4) could directly oxidize TFI/PDFI to produce strong CL emission in acidic solution. The effects of experimental conditions were investigated. Under the optimal conditions, the effect of albumin on the TFI/PDFI-KMnO(4) system was investigated. It was found that the addition of albumin into the system could induce enhancement of CL signal, and the enhanced CL intensity is linearly related to the logarithm of concentration of albumin. Based on this study, a novel CL method has been developed for the determination of albumin with high sensitivity and good selectivity. The method was applied to the determination of albumin in human serum samples, and the results were in agreement with those obtained by the bromcresol green (BCG) method. The relative errors for the analytical results were from -5.8% to 4.2%. These new phenomena would further enable people to exploit more CL analytical application of the heterocyclic imidazole derivatives.
在本文中，选择 2,4,5-三（2-呋喃基）-1H-咪唑（TFI）和 2-苯基-4,5-二（2-呋喃基）-1H-咪唑（PDFI）来研究 2-取代-4,5-二（2-呋喃基）-1H-咪唑的化学发光（CL）性质。详细研究了分析物被高锰酸钾（KMnO4）直接氧化的 CL。在酸性溶液中，KMnO4 可以直接将 TFI/PDFI 氧化为强 CL 发射。研究了实验条件的影响。在最佳条件下，研究了白蛋白对 TFI/PDFI-KMnO4 体系的影响。结果发现，向体系中加入白蛋白会诱导 CL 信号增强，并且增强的 CL 强度与白蛋白浓度的对数呈线性关系。基于这项研究，开发了一种用于高灵敏度和良好选择性测定白蛋白的新型 CL 方法。该方法用于测定人血清样品中的白蛋白，结果与溴甲酚绿（BCG）法一致。分析结果的相对误差为-5.8%至 4.2%。这些新现象将进一步使人们能够开发更多杂环咪唑衍生物的 CL 分析应用。
