Rice G E, Wong M H, Christensen P, Dantzer V, Skadhauge E
Department of Physiology, Monash University, Clayton, Vic. Australia.
Reprod Fertil Dev. 1990;2(5):581-6. doi: 10.1071/rd9900581.
The effects of porcine fetal fluids (amniotic and allantoic) on microsomal prostaglandin G/H synthase (PGHS) activity were assessed. Both amniotic and allantoic fluids obtained from late-gestation sows stimulated PGHS activity (as indicated by increased formation of radiolabelled prostaglandin) in a dose-dependent manner. At the maximum dose tested, amniotic and allantoic fluids stimulated prostaglandin (PG) formation by 55.5 +/- 1.5 and 58.5 +/- 4.7%, respectively (n = 3, P less than 0.01). Based upon ED50 values, amniotic fluid was approximately threefold more effective than allantoic fluid in stimulating PG formation. The stimulatory effect of amniotic but not allantoic fluid increased significantly (P less than 0.01) during gestation (Days 47-112). The observed changes in the stimulatory effect of amniotic fluid on microsomal PG formation parallels the in vivo changes that occur in intra-uterine PG synthesis. Amniotic fluid stimulatory activity may contribute to this gestational increase in PG synthesis.
评估了猪胎儿液体（羊水和尿囊液）对微粒体前列腺素G/H合酶（PGHS）活性的影响。从妊娠后期母猪获得的羊水和尿囊液均以剂量依赖性方式刺激PGHS活性（以放射性标记前列腺素形成增加表示）。在测试的最大剂量下，羊水和尿囊液分别刺激前列腺素（PG）形成55.5±1.5%和58.5±4.7%（n = 3，P<0.01）。根据半数有效剂量（ED50）值，羊水在刺激PG形成方面比尿囊液有效约三倍。在妊娠期间（第47 - 112天），羊水而非尿囊液的刺激作用显著增加（P<0.01）。观察到的羊水对微粒体PG形成刺激作用的变化与子宫内PG合成的体内变化相似。羊水刺激活性可能促成了PG合成的这种妊娠期间增加。
