Challis J R, Patrick J E, Cross J, Workewych J, Manchester E, Power S
Can J Physiol Pharmacol. 1981 Mar;59(3):261-7. doi: 10.1139/y81-041.
Fluctuations in the concentrations of cortisol and progesterone in fetal plasma, maternal plasma, and amniotic and allantoic fluids were measured in samples taken at 10-min intervals over a 90-min period from three groups of sheep sampled at different times during late pregnancy. During the last 30 days of gestation there was a significant rise in the mean concentration of cortisol in fetal plasma and amniotic fluid and a significant correlation between the cortisol concentration in these two fluids. The concentration of cortisol in allantoic fluid exceeded that in amniotic fluid. The concentration of cortisol in fetal plasma varied in a pulsatile manner, however the coefficient of variation (CV) within animals was greater (36%) on days -11 to -20, relative to the day of parturition (day 0), than on days -21 to -30 or days -5 to 0(15-19%). The CV values for cortisol in amniotic fluid and maternal plasma during the last 30 days of pregnancy were 20-50% and two at five times greater than the intraassay CV. The concentration of progesterone in amniotic fluid increased after day -20 but was not correlated with that in maternal plasma or fetal plasma. The concentrations of progesterone in paired samples of amniotic fluid and allantoic fluid were similar. The CV values for progesterone (18-34%) were similar in fetal and maternal plasma and amniotic fluid and did not change significantly during late pregnancy. Changes in the concentration of progesterone were unrelated to changes in cortisol. Interpretation of steroid profiles in fetal plasma and fluids through late pregnancy should take into account these short-term fluctuations in hormone concentrations.
在妊娠后期不同时间对三组绵羊进行取样，在90分钟内每隔10分钟采集一次样本，测量胎儿血浆、母体血浆、羊水和尿囊液中皮质醇和孕酮的浓度波动。在妊娠的最后30天，胎儿血浆和羊水中皮质醇的平均浓度显著升高，且这两种液体中皮质醇浓度之间存在显著相关性。尿囊液中皮质醇的浓度超过羊水中的浓度。胎儿血浆中皮质醇的浓度呈脉冲式变化，然而，相对于分娩日（第0天），在-11至-20天动物体内的变异系数（CV）（36%）高于-21至-30天或-5至0天（15 - 19%）。妊娠最后30天羊水中皮质醇和母体血浆中的CV值为20 - 50%，比测定内CV高两到五倍。羊水孕酮浓度在-20天后升高，但与母体血浆或胎儿血浆中的孕酮浓度无关。羊水和尿囊液配对样本中的孕酮浓度相似。胎儿和母体血浆以及羊水中孕酮的CV值（18 - 34%）相似，在妊娠后期无显著变化。孕酮浓度的变化与皮质醇的变化无关。在妊娠后期解释胎儿血浆和液体中的类固醇谱时应考虑到这些激素浓度的短期波动。
