一种从菠菜中分离光系统-I 膜复合物的新型膜基工艺。

A novel membrane based process to isolate photosystem-I membrane complex from spinach.

机构信息

Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, People's Republic of China.

出版信息

Photosynth Res. 2011 Feb;107(2):187-93. doi: 10.1007/s11120-011-9625-5. Epub 2011 Jan 28.

DOI:10.1007/s11120-011-9625-5

PMID:21274629

Abstract

The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O(2) electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone.

摘要

菠菜的光系统 I（PS-I）已通过使用 300 kDa 分子量截止聚醚砜膜的超滤进行分离。通过参数扫描超滤优化了超滤操作条件对 PS-I 活性的影响。这些条件包括溶液 pH 值、离子强度、搅拌速度和渗透通量。还使用 O(2)电极研究了去污剂（Triton X-100 和正十二烷基-β-D-麦芽糖苷）浓度对 PS-I 时变活性的影响。在优化条件下，在保留物中获得的 PS-I 纯度约为 84%，超滤后活性回收率大于 94%。据我们所知，这是首次单独使用超滤分离膜蛋白的报道。