Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi, 755-8505, Japan.
Inflamm Res. 2011 Jun;60(6):605-10. doi: 10.1007/s00011-011-0310-0. Epub 2011 Jan 28.
Human thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine, promotes inflammatory T helper type 2 cell (Th2) differentiation of naive CD4(+) T cells. TSLP is highly produced in keratinocytes of patients with atopic dermatitis and bronchial epithelia of patients with asthma and was thought to be a master switch for allergic inflammation. We sought to examine the effect of TSLP in human monocytes/macrophages.
The effect of TSLP on the expression of cell surface antigens (CD11c, CD16, CD54, CD80, CD86, and HLA-DR) in peripheral blood CD14(+) monocytes/macrophages was examined.
TSLP enhanced the expression of CD80 in peripheral blood CD14(+) monocytes/macrophages but not that of other cell surface antigens. It was associated with an increased percentage of CD14(dim/-), CD80(+), CD11c(+), and HLA-DR(+) cells, which was consistent with the increased differentiation of myeloid dendritic cells.
TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.
人胸腺基质淋巴细胞生成素（TSLP）是一种上皮细胞衍生的细胞因子，可促进初始 CD4+T 细胞向炎症性辅助性 T 细胞 2 型（Th2）分化。特应性皮炎患者的角质形成细胞和哮喘患者的支气管上皮细胞中高表达 TSLP，它被认为是过敏炎症的主要开关。我们试图研究 TSLP 在人单核细胞/巨噬细胞中的作用。
研究 TSLP 对人外周血 CD14+单核细胞/巨噬细胞表面抗原（CD11c、CD16、CD54、CD80、CD86 和 HLA-DR）表达的影响。
TSLP 增强了外周血 CD14+单核细胞/巨噬细胞中 CD80 的表达，但对其他表面抗原没有影响。它与 CD14(dim/-)、CD80(+)、CD11c(+)和 HLA-DR(+)细胞百分比的增加有关，这与髓样树突状细胞的分化增加一致。
TSLP 诱导人外周血 CD14+单核细胞/巨噬细胞中 CD80 的表达；这表明单核细胞/巨噬细胞被激活。这可能与其分化为髓样树突状细胞有关。
