Kunz F, Corti S, Giezendanner N, Stephan R, Wittenbrink M, Zweifel C
Institut für Lebensmittelsicherheit und -hygiene, Universität Zürich.
Schweiz Arch Tierheilkd. 2011 Feb;153(2):63-9. doi: 10.1024/0036-7281/a000152.
Staphylococcus aureus and coagulase negative staphylococci (CNS) were isolated from ovine and caprine mastitis milk samples originating from more than 40 Swiss farms. CNS dominated as causal microorganisms of mastitis in small ruminants. By restriction fragment length polymorphism (RFLP) analysis of the groEL gene and sequencing of 16S rDNA, various CNS species were identified, albeit certain of them predominated. For susceptibility testing of mastitis pathogens to selected antibiotics, minimal inhibitory concentrations were determined. Of the 67 S. aureus and 208 CNS strains, 31.3 % and 8.2 % were resistant to penicillin, 29.9 % and 1.0 % to ampicillin, 1.5 % and 10.6 % to erythromycin, and 3.0 % and 7.7 % to tetracycline, respectively. Moreover, 10 CNS strains (4.8 %) were resistant to oxacillin and one CNS strain to sulfamethoxazole/trimethoprim. The results obtained describe for the first time the resistance situation of mastitis pathogens from sheep and goats in Switzerland. However, accompanying and preventing measures are also of importance in mastitis control of small ruminants.
从瑞士40多个农场采集的绵羊和山羊乳腺炎乳样中分离出金黄色葡萄球菌和凝固酶阴性葡萄球菌（CNS）。CNS作为小反刍动物乳腺炎的致病微生物占主导地位。通过对groEL基因的限制性片段长度多态性（RFLP）分析和16S rDNA测序，鉴定出了多种CNS菌种，尽管其中某些菌种占主导。为了检测乳腺炎病原体对所选抗生素的敏感性，测定了最低抑菌浓度。在67株金黄色葡萄球菌和208株CNS菌株中，分别有31.3%和8.2%对青霉素耐药，29.9%和1.0%对氨苄青霉素耐药，1.5%和10.6%对红霉素耐药，3.0%和7.7%对四环素耐药。此外，10株CNS菌株（4.8%）对苯唑西林耐药，1株CNS菌株对磺胺甲恶唑/甲氧苄啶耐药。所获得的结果首次描述了瑞士绵羊和山羊乳腺炎病原体的耐药情况。然而，伴随措施和预防措施在小反刍动物乳腺炎控制中也很重要。
