Plant Physiology, Biochemistry, Molecular Biology Program, Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky 40546, USA.
Plant Cell Environ. 2011 May;34(5):811-20. doi: 10.1111/j.1365-3040.2011.02284.x. Epub 2011 Mar 15.
In spite of the mounting concerns, current understanding of the extent and mechanisms of phytotoxicity of manufactured nanomaterials remains limited. Here we show that in Arabidopsis thaliana, ultra-small anatase TiO(2) nanoparticles cause reorganization and elimination of microtubules followed by the accelerated and 26S proteasome-dependent degradation of tubulin monomers. Similar to other microtubule-disrupting agents, TiO(2) nanoparticles induce isotropic growth of root cells. Because microtubules are essential for the normal function of all eukaryotic cells, these results reveal a potentially important consequence of environmental pollution by this widely used nanomaterial.
尽管人们越来越关注，但目前对于制造纳米材料的植物毒性的程度和机制的了解仍然有限。在这里，我们表明在拟南芥中，超小锐钛矿 TiO(2)纳米颗粒导致微管的重排和消除，随后微管蛋白单体的降解加速并依赖于 26S 蛋白酶体。与其他微管破坏剂类似，TiO(2)纳米颗粒诱导根细胞的各向同性生长。由于微管对于所有真核细胞的正常功能都是必不可少的，因此这些结果揭示了这种广泛使用的纳米材料对环境污染的潜在重要后果。
