Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Int Endod J. 2011 Jun;44(6):499-504. doi: 10.1111/j.1365-2591.2011.01853.x. Epub 2011 Jan 28.
To determine mRNA expression levels of Nav 1.8 in inflamed pulps of rats.
Inflammation was induced by creating pulp exposures in rat incisors. Histopathological changes in the induced pulpitis were evaluated 1, 3, 7 and 10 days after exposure. Using real-time PCR, the relative mRNA expression levels of Nav 1.8 in the inflamed rat dental pulp was determined.
At day 1, no inflammation was evident in the pulp tissue, whereas increased levels of inflammatory responses were identified at day 3 and day 7. No pulpal inflammation was evident in day 10 or in the control group. Nav 1.8 was expressed in the rat dental pulp and increased at day 3 and day 7. Time course study of dental pulp inflammation indicated that differences in relative mRNA expression levels of Nav 1.8 were correlated with the severity of inflammation.
Nav 1.8 channels seem to be expressed significantly more under a temporal control so as to be associated with a severity of inflammation during pulpitis. As Nav 1.8 has been considered to have a role in neuropathic pain, its expression within dental pulp may contribute to the pathophysiology of tooth pain.
确定 Nav 1.8 在大鼠炎症牙髓中的 mRNA 表达水平。
通过在大鼠切牙上造成牙髓暴露来诱导炎症。在暴露后 1、3、7 和 10 天评估诱导性牙髓炎中的组织病理学变化。使用实时 PCR 确定炎症大鼠牙髓中 Nav 1.8 的相对 mRNA 表达水平。
在第 1 天，牙髓组织中没有明显的炎症，而在第 3 天和第 7 天则出现了炎症反应的增加。在第 10 天或对照组中没有明显的牙髓炎症。Nav 1.8 在大鼠牙髓中表达，并在第 3 天和第 7 天增加。牙髓炎症的时程研究表明，Nav 1.8 的相对 mRNA 表达水平的差异与炎症的严重程度相关。
Nav 1.8 通道似乎在时间控制下表达显著增加，与牙髓炎期间的炎症严重程度相关。由于 Nav 1.8 被认为在神经性疼痛中起作用，其在牙髓内的表达可能有助于牙齿疼痛的病理生理学。
