Fujioka A, Suya H, Pincelli C, Hashimoto A, Fukuyama K, Epstein W L
Department of Dermatology, University of California, San Francisco.
Nat Immun Cell Growth Regul. 1990;9(5):342-52.
We investigated the effect of granulomatous inflammation in skin on lymphocyte maturation in athymic (nu/nu) mice. Hepatic egg granulomas developed in euthymic (nu/+) mice with schistosomiasis were transplanted into skin of nu/nu mice. During skin granuloma development the rate of DNA synthesis and interleukin 2 activity of lymphocytes from lymph nodes, with and without concanavalin A stimulation, showed that the nu/nu cells were activated to levels of untreated nu/+ lymph node cells. Activation of splenic lymphocytes was not detected in the grafted nu/nu mice. Also, immunohistochemical staining demonstrated an increase in cells expressing Thy 1.2, Lyt-1 or L3T4 surface markers in the skin and lymph nodes, but not in spleen. The findings indicate that a granulomatous reaction in nu/nu mouse skin induces local, but not systemic, proliferation and differentiation of lymphocytes, to a low degree compatible with resting nu/+ mice.
我们研究了无胸腺（nu/nu）小鼠皮肤中的肉芽肿性炎症对淋巴细胞成熟的影响。将感染血吸虫病的正常胸腺（nu/+）小鼠所形成的肝虫卵肉芽肿移植到nu/nu小鼠的皮肤中。在皮肤肉芽肿形成过程中，无论有无伴刀豆球蛋白A刺激，来自淋巴结的淋巴细胞的DNA合成速率和白细胞介素2活性表明，nu/nu细胞被激活至未处理的nu/+淋巴结细胞的水平。在移植的nu/nu小鼠中未检测到脾淋巴细胞的激活。此外，免疫组织化学染色显示，皮肤和淋巴结中表达Thy 1.2、Lyt-1或L3T4表面标志物的细胞增加，但脾脏中未增加。这些发现表明，nu/nu小鼠皮肤中的肉芽肿反应诱导淋巴细胞局部而非全身的增殖和分化，其程度较低，与静止的nu/+小鼠相当。
