基于克拉克型过氧化氢或氧电极，利用核苷磷酸化酶-黄嘌呤氧化酶酶传感器安培法测定磷酸盐。
Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on a Clark-type hydrogen peroxide or oxygen electrode.
作者信息
Haemmerli S D, Suleiman A A, Guilbault G G
机构信息
University of New Orleans, Department of Chemistry, Louisiana 70148.
出版信息
Anal Biochem. 1990 Nov 15;191(1):106-9. doi: 10.1016/0003-2697(90)90395-p.
PMID:2127658
Abstract
A bienzymatic sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 microM.
摘要
通过将黄嘌呤氧化酶（EC 1.2.3.22）和核苷磷酸化酶（EC 2.4.2.1）共固定在安装在安培型过氧化氢和氧电极尖端的聚碳酸酯膜上，构建了一种用于测定磷酸盐的双酶传感器。该传感器对磷酸盐浓度在10 - 250微摩尔范围内呈线性响应。
相似文献
1
Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on a Clark-type hydrogen peroxide or oxygen electrode.基于克拉克型过氧化氢或氧电极，利用核苷磷酸化酶-黄嘌呤氧化酶酶传感器安培法测定磷酸盐。
Anal Biochem. 1990 Nov 15;191(1):106-9. doi: 10.1016/0003-2697(90)90395-p.
2
An FIA biosensor system for the determination of phosphate.一种用于测定磷酸盐的流动注射分析生物传感器系统。
Biosens Bioelectron. 1991;6(7):581-7. doi: 10.1016/0956-5663(91)80023-q.
3
Determination of phosphate ions with an enzyme sensor system.用酶传感器系统测定磷酸根离子。
Biosensors. 1987;3(5):297-306. doi: 10.1016/0265-928x(87)80002-0.
4
Determination of phosphate in nanomolar range by an enzyme-coupling fluorescent method.酶偶联荧光法测定纳摩尔范围内的磷酸盐
Anal Biochem. 2003 Sep 15;320(2):292-8. doi: 10.1016/s0003-2697(03)00400-7.
5
Determination of inorganic phosphate by flow injection method with immobilized enzymes and chemiluminescence detection.采用固定化酶和化学发光检测的流动注射法测定无机磷酸盐。
Anal Biochem. 1989 Nov 1;182(2):366-70. doi: 10.1016/0003-2697(89)90609-x.
6
Enzymic determination of inorganic phosphates, organic phosphates and phosphate-liberating enzymes by use of nucleoside phosphorylase-xanthine oxidase (dehydrogenase)-coupled reactions.利用核苷磷酸化酶-黄嘌呤氧化酶（脱氢酶）偶联反应对无机磷酸盐、有机磷酸盐及磷酸盐释放酶进行酶法测定。
Biochem J. 1985 Aug 15;230(1):255-60. doi: 10.1042/bj2300255.
7
Determination of plasma purine nucleoside phosphorylase activity by high-performance liquid chromatography.用高效液相色谱法测定血浆嘌呤核苷磷酸化酶活性
Anal Biochem. 1995 May 1;227(1):135-9. doi: 10.1006/abio.1995.1262.
8
Purine nucleoside phosphorylase in erythrocytes: determination of optimum reaction conditions.
Clin Biochem. 1989 Feb;22(1):3-9. doi: 10.1016/s0009-9120(89)80062-1.
9
A quantitative histochemical procedure for the demonstration of purine nucleoside phosphorylase activity in rat and human liver using Tetranitro BT and xanthine oxidase as auxiliary enzyme.一种使用四硝基BT和黄嘌呤氧化酶作为辅助酶来显示大鼠和人肝脏中嘌呤核苷磷酸化酶活性的定量组织化学方法。
Histochem J. 1993 Jan;25(1):86-91. doi: 10.1007/BF00161048.
10
An amperometric biosensor for fish freshness detection from xanthine oxidase immobilized in polypyrrole-polyvinylsulphonate film.一种用于检测鱼类新鲜度的安培型生物传感器，由固定在聚吡咯 - 聚乙烯磺酸盐薄膜中的黄嘌呤氧化酶构成。
Artif Cells Blood Substit Immobil Biotechnol. 2012 Aug;40(4):275-9. doi: 10.3109/10731199.2011.646410. Epub 2012 Jan 16.
Zotero 插件