基于克拉克型过氧化氢或氧电极，利用核苷磷酸化酶-黄嘌呤氧化酶酶传感器安培法测定磷酸盐。
Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on a Clark-type hydrogen peroxide or oxygen electrode.
作者信息
Haemmerli S D, Suleiman A A, Guilbault G G
机构信息
University of New Orleans, Department of Chemistry, Louisiana 70148.
出版信息
Anal Biochem. 1990 Nov 15;191(1):106-9. doi: 10.1016/0003-2697(90)90395-p.
PMID:2127658
Abstract
A bienzymatic sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 microM.
摘要
通过将黄嘌呤氧化酶（EC 1.2.3.22）和核苷磷酸化酶（EC 2.4.2.1）共固定在安装在安培型过氧化氢和氧电极尖端的聚碳酸酯膜上，构建了一种用于测定磷酸盐的双酶传感器。该传感器对磷酸盐浓度在10 - 250微摩尔范围内呈线性响应。
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