用酶传感器系统测定磷酸根离子。

Determination of phosphate ions with an enzyme sensor system.

作者信息

Watanabe E, Endo H, Toyama K

机构信息

Department of Food Science and Technology, Tokyo University of Fisheries, Japan.

出版信息

Biosensors. 1987;3(5):297-306. doi: 10.1016/0265-928x(87)80002-0.

DOI:10.1016/0265-928x(87)80002-0

PMID:3149200

Abstract

An enzyme sensor system for the determination of phosphate ions was constructed using immobilized enzymes and an oxygen electrode. The principle of this method is based upon the nucleoside phosphorylase catalyzed reaction for which the presence of inorganic phosphorus is indispensable. One assay could be completed within 3 min. This enzyme sensor was able to withstand at least 70 assays. This system was applicable to simple, rapid and continuous determination of phosphate ions in food.

摘要

利用固定化酶和氧电极构建了一种用于测定磷酸根离子的酶传感器系统。该方法的原理基于核苷磷酸化酶催化的反应，而无机磷的存在是必不可少的。一次测定可在3分钟内完成。这种酶传感器至少能够承受70次测定。该系统适用于食品中磷酸根离子的简单、快速和连续测定。