Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK.
Curr Opin Chem Biol. 2011 Apr;15(2):211-9. doi: 10.1016/j.cbpa.2010.12.002. Epub 2011 Jan 26.
Enzymes are able to perform a multitude of chemical and biochemical transformations with efficiencies that are typically unrivalled by chemical catalysts. However, these evolved systems may lack breadth or utility in other non-natural applications. Altering enzyme and protein scaffolds through covalent modification can expand the usefulness of native biocatalysts not only for synthetic application but also for therapeutic use. This review summarizes recent developments in the field of chemical modification of enzymes and how they can be applied to synthesis and biological research.
酶能够以通常优于化学催化剂的效率进行多种化学和生化转化。然而，这些进化系统在其他非自然应用中可能缺乏广度或实用性。通过共价修饰改变酶和蛋白质支架可以扩展天然生物催化剂的用途，不仅用于合成应用，也用于治疗用途。本文总结了酶化学修饰领域的最新进展以及它们如何应用于合成和生物研究。
