Dipartimento di Biologia e Biotecnologie Charles Darwin, Sapienza Università di Roma, Via dei Sardi 70, 00185 Rome, Italy.
Int J Med Microbiol. 2011 Apr;301(4):318-24. doi: 10.1016/j.ijmm.2010.11.005. Epub 2011 Jan 26.
Integrative conjugative elements (ICEs) of the SXT/R391 family are self-transmissible mobile elements mainly involved in antibiotic resistance spread among γ-Proteobacteria, including Vibrio cholerae. We demonstrated that the recently described ICEVchInd5 is prevailing in V. cholerae O1 clinical strains isolated in Wardha province (Maharashtra, India) from 1994 to 2005. Genetic characterization by ribotyping and multiple-locus SSR analysis proved the same clonal origin for V. cholerae O1 isolates in Wardha province over an 11-year period and was used to assess the correlation between strain and ICE content among ours and different Indian reference strains. In silico analysis showed the existence of at least 3 sibling ICEs of ICEVchInd5 in V. cholerae O1 El Tor reference strains, isolated in the Indian subcontinent after 1992.
SXT/R391 家族的整合共轭元件（ICEs）是自我可传输的移动元件，主要涉及γ-变形菌（包括霍乱弧菌）中的抗生素耐药性传播。我们证明，最近描述的 ICEVchInd5 在 1994 年至 2005 年期间从印度马哈拉施特拉邦沃德哈省分离的霍乱弧菌 O1 临床菌株中流行。通过核糖体分型和多位点 SSR 分析进行的遗传特征分析证明，在 11 年间，沃德哈省的霍乱弧菌 O1 分离株具有相同的克隆起源，并用于评估我们的菌株和不同印度参考菌株之间的菌株与 ICE 含量之间的相关性。计算机分析显示，在 1992 年后在印度次大陆分离的霍乱弧菌 O1 El Tor 参考菌株中，至少存在 3 个 ICEVchInd5 的姐妹 ICE。
