Kaas Rolf S, Ngandjio Antoinette, Nzouankeu Ariane, Siriphap Achiraya, Fonkoua Marie-Christine, Aarestrup Frank M, Hendriksen Rene S
National Food Institute, Technical University of Denmark, Research Group for Genomic Epidemiology, WHO Collaborating Center for Antimicrobial Resistance in Foodborne Pathogens and European Union Reference Laboratory for Antimicrobial Resistance, Kgs. Lyngby, Denmark.
Centre Pasteur du Cameroon, Service Hygiène et Environnement section Microbiologie, P.O. Box 1274, Yaoundé, Cameroon.
PLoS One. 2016 May 18;11(5):e0155691. doi: 10.1371/journal.pone.0155691. eCollection 2016.
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
直到1991年，乍得湖盆地周边报告的霍乱流行率相对较低。自那时起，每隔几年就有霍乱疫情报告。本研究的目的是调查2010/2011年喀麦隆的霍乱弧菌疫情，以深入了解引发该疫情的霍乱弧菌菌株的基因组构成。分离出24株菌株并进行全基因组测序。鉴定并注释了已知的毒力基因、抗性基因和整合接合元件（ICE）元件。使用单核苷酸多态性（SNP）分析推断出一个全球系统发育树（378个基因组）。发现喀麦隆疫情具有克隆性，且聚类于远离其他非洲菌株的位置。此外，一部分菌株含有在ICE元件中发现的一个缺失，该缺失导致抗性降低。这些结果表明霍乱弧菌在乍得湖盆地呈地方性流行，且与其他非洲菌株不同。
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