Scrascia Maria, Pugliese Nicola, Maimone Francesco, Mohamud Kadigia A, Grimont Patrick A D, Materu Sadiki F, Pazzani Carlo
Dipartimento di Genetica e Microbiologia, Università di Bari, Italy.
Int J Med Microbiol. 2009 Mar;299(3):203-7. doi: 10.1016/j.ijmm.2008.07.003. Epub 2008 Sep 5.
One hundred and three Vibrio cholerae O1 strains, selected to represent the cholera outbreaks which occurred in Somalia in 1998-1999, were characterized by random amplified polymorphic DNA patterns, ribotyping, and antimicrobial susceptibility. All strains showed a unique amplified DNA pattern and 2 closely related ribotypes (B5a and B8a), among which B5a was the more frequently identified. Ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim, conferred, except for spectinomycin, by a conjugative plasmid IncC. These findings indicated that the group of strains active in Somalia in the late 1990s had a clonal origin.
选取了103株霍乱弧菌O1菌株，以代表1998 - 1999年在索马里发生的霍乱疫情，通过随机扩增多态性DNA图谱、核糖体分型和抗菌药敏试验对其进行特征分析。所有菌株均呈现独特的扩增DNA图谱以及2种密切相关的核糖体分型（B5a和B8a），其中B5a的检出频率更高。91株菌株对氨苄西林、氯霉素、壮观霉素、链霉素、磺胺甲恶唑和甲氧苄啶耐药，除壮观霉素外，耐药性由接合质粒IncC介导。这些发现表明，20世纪90年代后期在索马里活跃的菌株群具有克隆起源。
