聚乙二醇沉淀法和超滤法检测巨肌酸激酶和巨淀粉酶。

Detection of macro-creatine kinase and macroamylase by polyethylene glycol precipitation and ultrafiltration methods.

机构信息

ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.

出版信息

Clin Chim Acta. 2011 Nov 20;412(23-24):2052-7. doi: 10.1016/j.cca.2011.01.023. Epub 2011 Jan 26.

DOI:10.1016/j.cca.2011.01.023

PMID:21276785

Abstract

BACKGROUND

Macroenzymes may cause elevations in serum enzyme activity. Macroenzymes are not common; however their detection is important because they cause diagnostic confusion and therapeutic errors.

METHODS

We analyzed 2 of the most prevalent macroenzymes in the literature, macro-creatine kinase (macro-CK) and macroamylase, using 2 methods for detection, polyethylene glycol (PEG) precipitation and ultrafiltration (UF). Enzyme measurements were made using a Roche Modular Analytics P analyzer. Imprecision was assessed using quality control material. We evaluated 125 samples from apparently healthy subjects to establish reference intervals. For macro-CK comparison, 94 samples with activities >200 U/l were analyzed with both PEG precipitation and UF and compared to electrophoresis. PEG precipitation and UF were compared for macroamylase detection using 130 samples with amylase activities >110 U/l.

RESULTS

UF was more precise and demonstrated narrower reference intervals for both analytes. PEG precipitation and UF were able to detect true cases of macro-CK with overall agreement with electrophoresis of 79.8% and 80.9%, respectively. Both methods detected the same number of 'positive' macroamylase samples; however PEG precipitation resulted in a greater number of 'indeterminate' cases.

CONCLUSION

This is the first report where UF has been shown useful for the detection of both macro-CK and macroamylase.

摘要

背景

巨酶可能导致血清酶活性升高。巨酶并不常见；然而，它们的检测很重要，因为它们会导致诊断混淆和治疗错误。

方法

我们使用两种检测方法——聚乙二醇（PEG）沉淀法和超滤法（UF），分析了文献中最常见的两种巨酶，即巨肌酸激酶（macro-CK）和巨淀粉酶。使用罗氏 Modular Analytics P 分析仪进行酶测定。使用质控材料评估不精密度。我们评估了 125 名来自健康受试者的样本，以建立参考区间。为了比较 macro-CK，我们分析了 94 例活性>200 U/l 的样本，分别用 PEG 沉淀法和 UF 法与电泳法进行比较。使用 130 例淀粉酶活性>110 U/l 的样本，比较了 PEG 沉淀法和 UF 法检测巨淀粉酶的效果。