Levitt M D, Ellis C
Gastroenterology. 1982 Aug;83(2):378-82.
Macromolecules are known to precipitate selectively in concentrated solutions of polyethylene glycol. This report describes the use of polyethylene glycol 6000 to distinguish macroamylase from normal-sized serum amylase. Preliminary studies indicated that a PEG concentration of 12% and a 10-min incubation at 37 degrees C separated normal serum amylase from macroamylase. Using these conditions, study of 18 macroamylase-containing sera showed precipitation of at least 73% of the amylase activity. In contrast, in 46 normal sera and 16 hyperamylasemic (but not macroamylasemic) sera, less than 52% of the amylase activity was precipitated by polyethylene glycol. This test provides a rapid, simple, and accurate means of determining if macroamylasemia is the cause of hyperamylasemia.
已知大分子物质在聚乙二醇浓溶液中会选择性沉淀。本报告描述了使用聚乙二醇6000来区分巨淀粉酶与正常大小的血清淀粉酶。初步研究表明，12%的聚乙二醇浓度以及在37℃孵育10分钟可将正常血清淀粉酶与巨淀粉酶分离。使用这些条件，对18份含巨淀粉酶的血清进行研究，结果显示至少73%的淀粉酶活性出现沉淀。相比之下，在46份正常血清和16份高淀粉酶血症（但不含巨淀粉酶）血清中，聚乙二醇沉淀的淀粉酶活性不到52%。该检测为确定高淀粉酶血症是否由巨淀粉酶血症引起提供了一种快速、简单且准确的方法。
