Watanabe S, Kano U, Ito T, Kakehashi R, Ikoma J, Murata T, Suzuki S
Third Department of Internal Medicine, Mie University School of Medicine, Japan.
Hepatogastroenterology. 1990 Dec;37 Suppl 2:126-9.
Serum DNA polymerase activity (DNA-P) was detected in 27.6 per cent of non-A, non-B (NANB) hepatitis patients, 8.7 per cent of patients with alcoholic liver disease (ALD), 8.6 per cent of hepatitis B surface antigen (HBsAg)-positive patients and 19.0 per cent of HBsAg-negative blood donors with elevated serum glutamic-pyruvic transaminase (SGPT) concentrations. In contrast, none of the patients with hepatitis A, drug-induced liver injury or non-alcoholic fatty liver had DNA-P in their sera in the acute phase of the illness. All HBsAg-positive samples with detectable DNA-P were strongly positive for hepatitis B virus (HBV) DNA, but the samples from patients with NANB hepatitis and ALD and HBsAg-negative blood donors had no HBV DNA. Sensitivity to actinomycin D showed the heterogeneity of DNA-Ps in HBsAg-negative blood donors; the enzyme activity of one type was inhibited by 100 micrograms/ml of actinomycin D, whereas the other was not. The preference for exogenous template primers of these DNA-Ps was different to those of HBV and human retroviruses. The results reveal the prevalence of serum DNA-P in NANB hepatitis patients and suggest that two distinct agents are relevant to the aetiology of NANB hepatitis.
在27.6%的非甲非乙型（NANB）肝炎患者、8.7%的酒精性肝病（ALD）患者、8.6%的乙型肝炎表面抗原（HBsAg）阳性患者以及19.0%血清谷丙转氨酶（SGPT）浓度升高的HBsAg阴性献血者中检测到血清DNA聚合酶活性（DNA-P）。相比之下，甲型肝炎、药物性肝损伤或非酒精性脂肪肝患者在疾病急性期血清中均未检测到DNA-P。所有检测到DNA-P的HBsAg阳性样本中乙型肝炎病毒（HBV）DNA均呈强阳性，但NANB肝炎患者、ALD患者及HBsAg阴性献血者的样本中未检测到HBV DNA。对放线菌素D的敏感性显示HBsAg阴性献血者中DNA-P存在异质性；一种类型的酶活性可被100微克/毫升放线菌素D抑制，而另一种则不受影响。这些DNA-P对外源模板引物的偏好与HBV和人类逆转录病毒不同。结果揭示了NANB肝炎患者血清DNA-P的流行情况，并表明两种不同的病原体与NANB肝炎的病因相关。
