Zhou Y, Fukuoka M, Tanaka A
Division of Biological Chemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
J Appl Toxicol. 1990 Dec;10(6):447-53. doi: 10.1002/jat.2550100612.
A single oral dose of di-n-butyl phthalate (DBP) to male rats caused a sloughing of the germ cells at 6 h, with more severe sloughing at 24 and 48 h. DBP is metabolized to mono-n-butyl phthalate (MBP), which is transported through the blood-tubular barrier into the seminiferous lumen. MBP is incorporated into the lumen at a maximum rate between 1 and 3 h after dosing with DBP. MBP caused decreases in the activities of succinate dehydrogenase in the Sertoli cells and sorbitol dehydrogenase in the germ cells, an increase in the activity of lactate dehydrogenase (LDH) in the germ cells and in the seminiferous lumen and a decrease in testicular iron levels.
给雄性大鼠单次口服邻苯二甲酸二正丁酯（DBP）后，6小时时生殖细胞出现脱落，24小时和48小时时脱落更严重。DBP代谢为邻苯二甲酸单正丁酯（MBP），后者通过血 - 小管屏障转运至生精小管管腔。给药DBP后1至3小时，MBP以最大速率进入管腔。MBP导致支持细胞中琥珀酸脱氢酶以及生殖细胞中山梨醇脱氢酶的活性降低，生殖细胞和生精小管管腔中乳酸脱氢酶（LDH）的活性增加，并且睾丸铁水平降低。
