Fukuoka M, Zhou Y, Tanaka A, Ikemoto I, Machida T
Division of Biological Chemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
J Appl Toxicol. 1990 Aug;10(4):285-93. doi: 10.1002/jat.2550100410.
A single oral dose of di-n-butyl phthalate (DBP) to male rats caused histologically a sloughing of the germ cells at 6 h. On Days 1 and 2 more severe sloughing was seen, followed by atrophy and the dissociation of the germ cells from the Sertoli cells and the spermatogonia. Biochemically, there was elevation of gamma-glutamyl transferase, a decrease in sorbitol levels at 3 h and a decrease in the activity of aldose reductase at 6 h, in the testes of treated rats. This was followed by decreases in fructose levels and increases in the activity of lactate dehydrogenase (LDH) and in lactate levels at 12 h, and decreases in the activities of sorbitol dehydrogenase and succinate dehydrogenase on Day 2. LDH isoenzymes 4 and 5 increased at 6 h prior to the increase in lactate levels. Increases in the levels of inositol and the activities of alkaline phosphatase and lactate dehydrogenase were also observed. Thus, these data suggest that DBP-induced testicular toxicity is caused by a shortage of energy fuels from glucose metabolism or by an anoxia.
给雄性大鼠单次口服邻苯二甲酸二正丁酯（DBP）后，组织学检查显示6小时时生殖细胞出现脱落。在第1天和第2天，可见更严重的脱落，随后是生殖细胞萎缩以及生殖细胞与支持细胞和精原细胞分离。生化方面，处理组大鼠睾丸中的γ-谷氨酰转移酶升高，3小时时山梨醇水平降低，6小时时醛糖还原酶活性降低。随后，12小时时果糖水平降低，乳酸脱氢酶（LDH）活性和乳酸水平升高，第2天时山梨醇脱氢酶和琥珀酸脱氢酶活性降低。乳酸水平升高前6小时，LDH同工酶4和5增加。还观察到肌醇水平升高以及碱性磷酸酶和乳酸脱氢酶活性增加。因此，这些数据表明DBP诱导的睾丸毒性是由葡萄糖代谢产生的能量燃料短缺或缺氧引起的。
