Perchellet J P, Perchellet E M, Belman S
Division of Biology, Kansas State University, Manhattan 66506.
Nutr Cancer. 1990;14(3-4):183-93. doi: 10.1080/01635589009514093.
A single 2-mg dose of garlic oil applied 30 minutes before a single carcinogenic dose of 7,12-dimethylbenz[a]-anthracene (DMBA) inhibited papilloma production in Sencar mice. The three groups were controls (Group 1), garlic oil applied 30 minutes before DMBA (Group 2), and garlic oil applied 30 minutes after DMBA (Group 3). The percents of mice with papillomas at 20 weeks were 94, 72, and 79, respectively. The decreases in Groups 2 and 3 were significant. The number of papillomas per mouse was 4.2 +/- 0.5 (Group 1), 2.3 +/- 0.8 (Group 2), and 3.4 +/- 0.6 (Group 3). The decrease in Group 2 was significant. A single 5-mg dose of garlic oil maximally inhibited DMBA-induced epidermal DNA synthesis by 86% when applied two hours before the carcinogen. Two-stage promotion in DMBA-initiated Sencar mice was achieved by twice-weekly applications of 8.5 nmol of 12-O-tetradecanoylphorbol-13-acetate (TPA) for 2 weeks followed by twice-weekly applications of 8.5 nmol of mezerein for 18 weeks. The oils were applied 30 minutes after each promotion by TPA or mezerein. Single doses of 1 mg onion or garlic oil inhibited the first and second stages of promotion. The groups used were control (Group 1), garlic oil applied after stage 1 (Group 2), onion oil applied after stage 1 (Group 3), propenyl sulfide applied after stage 1 (Group 4), garlic oil applied after stage 2 (Group 5), onion oil applied after stage 2 (Group 6), and propenyl sulfide applied after stage 2 (Group 7). The percent of mice with papillomas was significantly decreased by all agents in Groups 2-7. The data are 81, 83, 91, 68, 96, and 86, respectively. The number of papillomas per mouse was significantly reduced by onion and garlic oils but not by propenyl sulfide. The data are 9.4 +/- 0.8, 6.3 +/- 0.7, 7.4 +/- 0.5, 9.2 +/- 1.2, 3.7 +/- 0.9, 6.2 +/- 0.6, and 9.1 +/- 1.4 for Groups 1-7, respectively. Onion and garlic oils inhibited the TPA-stimulated DNA synthesis when given as single doses of 5 mg one hour before TPA. The inhibition by garlic oil was most effective when given one hour before TPA but was evident when given from two hours before to two hours after TPA. These results, and those of others (AS Sadhana, Cancer Lett, 40, 193-197, 1988), who obtained inhibition of initiation, indicate that onion and garlic oils inhibit all stages of mouse skin tumorigenesis.(ABSTRACT TRUNCATED AT 400 WORDS)
在给致癌剂量的7,12 - 二甲基苯并[a]蒽（DMBA）前30分钟，单次涂抹2毫克大蒜油可抑制Sencar小鼠乳头瘤的产生。三组分别为对照组（第1组）、在DMBA前30分钟涂抹大蒜油的组（第2组）以及在DMBA后30分钟涂抹大蒜油的组（第3组）。20周时出现乳头瘤的小鼠百分比分别为94%、72%和79%。第2组和第3组的百分比下降具有显著性。每只小鼠的乳头瘤数量为4.2±0.5（第1组）、2.3±0.8（第2组）和3.4±0.6（第3组）。第2组的数量减少具有显著性。在致癌物前两小时单次涂抹5毫克大蒜油可使DMBA诱导的表皮DNA合成最大程度地抑制86%。在DMBA启动的Sencar小鼠中，通过每周两次涂抹8.5纳摩尔的12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）持续2周，然后每周两次涂抹8.5纳摩尔的大戟二萜醇酯持续18周来实现两阶段促癌。在每次用TPA或大戟二萜醇酯促癌后30分钟涂抹油剂。单剂量的1毫克洋葱或大蒜油可抑制促癌的第一和第二阶段。所用组分别为对照组（第1组）、在第一阶段后涂抹大蒜油的组（第2组）、在第一阶段后涂抹洋葱油的组（第3组）、在第一阶段后涂抹丙烯基硫醚的组（第4组）、在第二阶段后涂抹大蒜油的组（第5组）、在第二阶段后涂抹洋葱油的组（第6组）以及在第二阶段后涂抹丙烯基硫醚的组（第7组）。第2 - 7组中所有药剂均使出现乳头瘤的小鼠百分比显著降低。数据分别为81%、83%、91%、68%、96%和86%。每只小鼠的乳头瘤数量被洋葱油和大蒜油显著减少，但未被丙烯基硫醚减少。第1 - 7组的数据分别为9.4±0.8、6.3±0.7、7.4±0.5、9.2±1.2、3.7±0.9、6.2±0.6和9.1±1.4。当在TPA前一小时单次给予5毫克时，洋葱油和大蒜油可抑制TPA刺激的DNA合成。大蒜油在TPA前一小时给予时抑制效果最显著，但在TPA前两小时至后两小时给予时也有明显效果。这些结果以及其他研究（AS Sadhana，《癌症通讯》，40，193 - 197，1988）中获得的起始抑制结果表明，洋葱油和大蒜油可抑制小鼠皮肤肿瘤发生的所有阶段。（摘要截取自400字）
