Elliott Kathryn F, Rand Jacquie S, Fleeman Linda M, Morton John M, Markwell Peter J
Centre for Companion Animal Health, School of Veterinary Science, University of Queensland, St Lucia, QLD 4072, Australia.
Am J Vet Res. 2011 Feb;72(2):161-8. doi: 10.2460/ajvr.72.2.161.
To develop a standardized meal challenge test by assessing associations between food-withheld preprandial (ie, fasting) and postprandial triglyceride concentrations, determining the most appropriate sampling time to detect the peak concentration (highest postprandial concentration), and estimating reference intervals for fasting and postprandial concentrations in healthy dogs.
12 lean healthy mixed-breed dogs.
Dogs were fed a dry commercially available diet (fat, 31% metabolizable energy) for 3 weeks. After food was withheld for 23 to 24 hours, plasma triglyceride concentrations were measured 1 and 0.083 hours before and 1, 2, 3, 4, 5, 6, 9, and 12 hours after feeding of a standardized challenge meal (median amount eaten, 63 kcal/kg [127 kcal/kg⁰.⁷⁵]). Correlation and agreement between concentrations at peak and other time points were assessed by use of correlation coefficients and Bland-Altman limits of agreement. Reference intervals were calculated by use of a robust method.
Fasting and peak triglyceride concentrations were not closely associated. The highest concentration among samples obtained 2, 5, and 6 hours after meal consumption had closest agreement with peak concentration. In 5 of 12 dogs, concentrations 12 hours after eating were still significantly above baseline concentration (mean of each dog's fasting concentrations).
Fasting triglyceride concentration could not be used to accurately predict peak concentration. When estimating peak concentration, multiple samples should be collected 2, 5, and 6 hours after consumption of a standardized meal. Food may need to be withheld for > 12 hours when assessing fasting concentrations in healthy dogs.
通过评估餐前（即禁食）和餐后甘油三酯浓度之间的关联，确定检测峰值浓度（餐后最高浓度）的最合适采样时间，并估计健康犬禁食和餐后浓度的参考区间，来开发一种标准化的膳食激发试验。
12只健康的瘦型混血犬。
给犬喂食市售干粮（脂肪，占可代谢能量的31%），持续3周。禁食23至24小时后，在喂食标准化激发餐（中位摄入量，63千卡/千克[127千卡/千克⁰.⁷⁵]）前1小时和0.083小时以及喂食后1、2、3、4、5、6、9和12小时测量血浆甘油三酯浓度。通过使用相关系数和Bland-Altman一致性界限评估峰值浓度与其他时间点浓度之间的相关性和一致性。使用稳健方法计算参考区间。
禁食甘油三酯浓度与峰值浓度没有密切关联。餐后2、5和6小时采集的样本中，最高浓度与峰值浓度最接近。在12只犬中的5只中，进食后12小时的浓度仍显著高于基线浓度（每只犬禁食浓度的平均值）。
禁食甘油三酯浓度不能用于准确预测峰值浓度。估计峰值浓度时，应在食用标准化餐后2、5和6小时采集多个样本。评估健康犬的禁食浓度时，可能需要禁食超过12小时。
