Lim W H, Stewart A G
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Agents Actions. 1990 Nov;31(3-4):290-7. doi: 10.1007/BF01997622.
We have examined species differences in the mobilization of arachidonic acid and generation of prostacyclin in non-activated and activated peritoneal macrophages in vitro. Mobilization of 3H-arachidonic acid was reduced in rat activated macrophages compared with that in non-activated macrophages, but a similar difference was not observed in guinea-pig macrophages. In guinea-pig peritoneal macrophages, exposure to formyl-Methionyl-Leucyl-Phenylalanine (fMLP), platelet-activating factor (Paf), zymosan and A23187 increased the generation of prostacyclin. In contrast, in rat peritoneal macrophages, fMLP and Paf did not stimulate the mobilization of arachidonic acid or the generation of prostacyclin, whereas both zymosan and A23187 were effective stimuli. Pretreatment of either rats or guinea-pigs by intraperitoneal injection of C. Parvum reduced prostacyclin generation by peritoneal macrophages in vitro. We conclude that there may be species differences in receptor populations between guinea-pig and rat peritoneal macrophages. However, the reduction in eicosanoid generation induced by the inflammatory stimulus, C. Parvum is not species-dependent.
我们研究了体外未激活和激活的腹腔巨噬细胞中花生四烯酸动员和前列环素生成的种属差异。与未激活的巨噬细胞相比，大鼠激活的巨噬细胞中3H-花生四烯酸的动员减少，但豚鼠巨噬细胞中未观察到类似差异。在豚鼠腹腔巨噬细胞中，暴露于甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）、血小板活化因子（Paf）、酵母聚糖和A23187会增加前列环素的生成。相反，在大鼠腹腔巨噬细胞中，fMLP和Paf不会刺激花生四烯酸的动员或前列环素的生成，而酵母聚糖和A23187都是有效的刺激物。通过腹腔注射微小隐孢子虫对大鼠或豚鼠进行预处理，可降低体外腹腔巨噬细胞中前列环素的生成。我们得出结论，豚鼠和大鼠腹腔巨噬细胞之间的受体群体可能存在种属差异。然而，炎症刺激物微小隐孢子虫诱导的类花生酸生成减少并不依赖于种属。
