大鼠腹膜白细胞中花生四烯酸代谢的5-脂氧合酶和环氧化酶途径的差异调节
Differential regulation of 5-lipoxygenase and cyclo-oxygenase pathways of arachidonate metabolism in rat peritoneal leukocytes.
作者信息
Moroney M A, Forder R A, Carey F, Hoult J R
机构信息
King's College London.
出版信息
Br J Pharmacol. 1990 Sep;101(1):128-32. doi: 10.1111/j.1476-5381.1990.tb12101.x.
Abstract
- Simultaneous activation of the 5-lipoxygenase and cyclo-oxygenase pathways of arachidonate metabolism in rat peritoneal mixed leukocytes in response to A23187, chemoattractant N-formyl-methionine-leucine-phenylalanine (FMLP) and arachidonic acid (AA) was studied by radioimmunoassay of leukotriene B4 (LTB4) and thromboxane B2 (TXB2) respectively. 2. FMLP and AA preferentially activated cyclo-oxygenase and A23187 preferentially activated 5-lipoxygenase. Release of TXB2 preceded that of LTB4. A threshold amount of A23187 enhanced FMLP-and AA-induced LTB4 production but not that of TXB2. 3. Phorbol myristate acetate (PMA) abolished LTB4 generation in response to FMLP with much less effect on TXB2, but did not inhibit the formation of either eicosanoid caused by A23187 or AA. Instead, PMA caused a dose-dependent but modest stimulation of TXB2 generation either on its own or when added with A23187 or AA. 4. These results show that the 5-lipoxygenase and cyclo-oxygenase pathways in rat peritoneal leukocytes are regulated differently and that functional compartmentalisation of the stimulus-generation sequence operates in these cells.
摘要
- 通过分别对白三烯B4（LTB4）和血栓素B2（TXB2）进行放射免疫测定，研究了大鼠腹膜混合白细胞中花生四烯酸代谢的5-脂氧合酶和环氧化酶途径在响应A23187、趋化因子N-甲酰甲硫氨酸-亮氨酸-苯丙氨酸（FMLP）和花生四烯酸（AA）时的同时激活情况。2. FMLP和AA优先激活环氧化酶，而A23187优先激活5-脂氧合酶。TXB2的释放先于LTB4。一定阈值量的A23187可增强FMLP和AA诱导的LTB4生成，但不增强TXB2的生成。3. 佛波酯（PMA）消除了FMLP诱导的LTB4生成，对TXB2的影响小得多，但不抑制A23187或AA引起的任何一种类二十烷酸的形成。相反，PMA单独或与A23187或AA一起添加时，会引起TXB2生成的剂量依赖性但适度的刺激。4. 这些结果表明，大鼠腹膜白细胞中的5-脂氧合酶和环氧化酶途径受到不同的调节，并且刺激产生序列的功能区室化在这些细胞中起作用。
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