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[Preparative representation of the living eggs of Schistosoma mansoni].

作者信息

Schlisio W, Lafrenz M

机构信息

Abteilung für Tropenmedizin und Infektionskrankheiten, Universität Rostock.

出版信息

Angew Parasitol. 1990 Nov;31(4):203-6.

PMID:2128171
Abstract

To maintain the hatchability of miracidia, pure worm eggs of S. mansoni were collected in great quantities from liver and spleen of experimentally infected mice. For this purpose, the tissue was digested with papain, hyaluronidase and trypsin. Worm eggs in the residue were separated from remaining host cells, tissue materials and dead eggs by using the sieving chamber (sieves 200 microns x 200 microns and 28 microns x 28 microns) and by density gradient centrifugation.

摘要

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