Centre Scientifique de Monaco, Avenue Saint-Martin, Monaco.
Comp Biochem Physiol B Biochem Mol Biol. 2011 May;159(1):40-8. doi: 10.1016/j.cbpb.2011.01.007. Epub 2011 Jan 28.
We analysed the soluble organic matrix (SOM) of two biominerals formed by the same organism but differing by their morphological characteristics: the axial skeleton and the sclerites of Corallium rubrum. The results of 1D SDS-PAGE electrophoresis show for the two biominerals that SOM proteins bands have similar apparent molecular weight but differ in quantity. Further analysis by 2D electrophoresis reveals each protein band as a line of spots with different isoelectric points. Our results suggest that each SOM protein band consists of a mix of proteins and/or one unique protein with post-translational modifications. By immunohistochemistry, we show that antibodies raised against the SOM of axial skeleton and sclerites label the SOM of the two biominerals but also label the insoluble organic matrix suggesting the presence of common epitopes between the two biominerals and the two organic fractions.
我们分析了由同一生物体形成的两种生物矿物的可溶性有机基质（SOM），但它们的形态特征不同：轴向骨骼和红珊瑚的骨板。1D SDS-PAGE 电泳的结果表明，两种生物矿物的 SOM 蛋白条带具有相似的表观分子量，但在数量上有所不同。通过二维电泳进一步分析表明，每个蛋白条带都是一条具有不同等电点的斑点线。我们的结果表明，每个 SOM 蛋白条带由蛋白质混合物和/或一种具有翻译后修饰的独特蛋白质组成。通过免疫组织化学，我们表明针对轴向骨骼和骨板 SOM 制备的抗体标记两种生物矿物的 SOM，也标记不溶性有机基质，这表明两种生物矿物和两种有机部分之间存在共同的表位。
