Mikhaĭlov A D, Zhordaniia K I
Biokhimiia. 1990 Nov;55(11):2103-7.
Human galactosyl transferase (EC 2.4.1.38), a potential tumour marker, was isolated from the ascite fluid of patients with ovary cancer by three methods based on the use of affinity adsorbents. The highest degree of purification was achieved during chromatography on galactosyl transferase-specific monoclonal antibodies immobilized on agarose. A comparison of physico-chemical properties of purified preparations was carried out. It was found that the enzyme purified on the immunoadsorbent did not practically differ in terms of molecular mass and isospectra from other enzyme preparations, which suggests that the antigenic epitope recognized by these monoclonal antibodies on the galactosyl transferase molecule is common for all enzyme isoforms.
人半乳糖基转移酶（EC 2.4.1.38）是一种潜在的肿瘤标志物，采用基于亲和吸附剂的三种方法，从卵巢癌患者的腹水中分离得到。在固定于琼脂糖上的半乳糖基转移酶特异性单克隆抗体上进行层析时，获得了最高的纯化程度。对纯化制剂的物理化学性质进行了比较。结果发现，在免疫吸附剂上纯化的酶在分子量和等谱方面与其他酶制剂几乎没有差异，这表明这些单克隆抗体在半乳糖基转移酶分子上识别的抗原表位对所有酶同工型来说是共同的。
