肺炎克雷伯菌 LamB 孔蛋白在抗菌药物耐药性中的作用。
Role of Klebsiella pneumoniae LamB Porin in antimicrobial resistance.
机构信息
Instituto Universitario de Investigaciones en Ciencias de Salud, Universidad de Islas Baleares, Palma de Mallorca, Spain.
出版信息
Antimicrob Agents Chemother. 2011 Apr;55(4):1803-5. doi: 10.1128/AAC.01441-10. Epub 2011 Jan 31.
Abstract
To investigate the contribution of LamB in Klebsiella pneumoniae antimicrobial resistance, we determined the MICs of various antibiotics and the frequency of mutation to increased cefoxitin or meropenem resistance of the strains CSUB10S (expressing only OmpK36), CSUB10R (lacking OmpK35 and OmpK36), and their derived isogenic insertion-duplication mutants deficient in LamB. Expression of LamB was indispensable in order for CSUB10S to lose OmpK36 and become resistant to cefoxitin, while in CSUB10R, LamB deficiency promoted increased resistance to carbapenem.
摘要
为了研究 LamB 在肺炎克雷伯菌抗药性中的作用，我们测定了各抗生素的 MIC 值以及 CSUB10S 株（仅表达 OmpK36）、CSUB10R 株（缺乏 OmpK35 和 OmpK36）及其衍生的同源插入缺失突变体对增加头孢西丁或美罗培南耐药性的突变频率。LamB 的表达对于 CSUB10S 丢失 OmpK36 并对头孢西丁产生耐药性是不可或缺的，而在 CSUB10R 中，LamB 缺失促进了对碳青霉烯类药物的耐药性增加。
相似文献
1
Role of Klebsiella pneumoniae LamB Porin in antimicrobial resistance.肺炎克雷伯菌 LamB 孔蛋白在抗菌药物耐药性中的作用。
Antimicrob Agents Chemother. 2011 Apr;55(4):1803-5. doi: 10.1128/AAC.01441-10. Epub 2011 Jan 31.
2
Global Trends in Proteome Remodeling of the Outer Membrane Modulate Antimicrobial Permeability in Klebsiella pneumoniae.全球趋势在蛋白质组重塑的外膜调节抗菌通透性在肺炎克雷伯菌。
mBio. 2020 Apr 14;11(2):e00603-20. doi: 10.1128/mBio.00603-20.
3
OmpK26, a novel porin associated with carbapenem resistance in Klebsiella pneumoniae.OmpK26，一种与肺炎克雷伯菌碳青霉烯类耐药相关的新型孔蛋白。
Antimicrob Agents Chemother. 2011 Oct;55(10):4742-7. doi: 10.1128/AAC.00309-11. Epub 2011 Aug 1.
4
Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence.肺炎克雷伯菌外膜孔蛋白 OmpK35 和 OmpK36 既参与抗菌药物耐药性的形成，也与毒力有关。
Antimicrob Agents Chemother. 2011 Apr;55(4):1485-93. doi: 10.1128/AAC.01275-10. Epub 2011 Jan 31.
5
Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production.产碳青霉烯酶肺炎克雷伯菌与孔蛋白 OmpK36 缺失和 DHA-1 型β-内酰胺酶产生相关，表现出对碳青霉烯类和头孢西丁的耐药性。
Braz J Microbiol. 2013 Oct 30;44(2):435-42. doi: 10.1590/S1517-83822013000200015. eCollection 2013.
6
Acquisition of carbapenem resistance in multiresistant Klebsiella pneumoniae strains harbouring blaCTX-M-15, qnrS1 and aac(6')-Ib-cr genes.产 blaCTX-M-15、qnrS1 和 aac(6')-Ib-cr 基因的多重耐药肺炎克雷伯菌中碳青霉烯类耐药的获得。
J Med Microbiol. 2012 May;61(Pt 5):672-677. doi: 10.1099/jmm.0.038083-0. Epub 2012 Jan 26.
7
Role of association of OmpK35 and OmpK36 alteration and bla and/or bla genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae.OmpK35 和 OmpK36 改变与 bla 和/或 bla 基因的关联在非碳青霉烯酶产生肺炎克雷伯菌中赋予碳青霉烯类耐药的作用。
Int J Antimicrob Agents. 2018 Dec;52(6):898-905. doi: 10.1016/j.ijantimicag.2018.03.020. Epub 2018 Apr 2.
8
An ertapenem-resistant extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae clone carries a novel OmpK36 porin variant.产厄他培南耐药超广谱β-内酰胺酶的肺炎克雷伯菌克隆携带一种新型的 OmpK36 孔蛋白变体。
Antimicrob Agents Chemother. 2010 Oct;54(10):4178-84. doi: 10.1128/AAC.01301-09. Epub 2010 Jul 26.
9
Expression of SHV-2 beta-lactamase and of reduced amounts of OmpK36 porin in Klebsiella pneumoniae results in increased resistance to cephalosporins and carbapenems.肺炎克雷伯菌中SHV-2β-内酰胺酶的表达以及外膜孔蛋白OmpK36表达量的减少，导致对头孢菌素和碳青霉烯类药物的耐药性增加。
Antimicrob Agents Chemother. 2002 Nov;46(11):3679-82. doi: 10.1128/AAC.46.11.3679-3682.2002.
10
Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance.肺炎克雷伯菌外膜孔蛋白OmpK35在抗菌耐药性中的作用。
Antimicrob Agents Chemother. 2003 Oct;47(10):3332-5. doi: 10.1128/AAC.47.10.3332-3335.2003.
引用本文的文献
1
Interplay between porin deficiency, fitness, and virulence in carbapenem-non-susceptible Pseudomonas aeruginosa and Enterobacteriaceae.碳青霉烯不敏感的铜绿假单胞菌和肠杆菌科细菌中孔蛋白缺乏、适应性与毒力之间的相互作用
PLoS Pathog. 2025 Feb 7;21(2):e1012902. doi: 10.1371/journal.ppat.1012902. eCollection 2025 Feb.
2
Efflux Pumps and Porins Enhance Bacterial Tolerance to Phenolic Compounds by Inhibiting Hydroxyl Radical Generation.外排泵和孔蛋白通过抑制羟基自由基的产生增强细菌对酚类化合物的耐受性。
Microorganisms. 2025 Jan 18;13(1):202. doi: 10.3390/microorganisms13010202.
3
Genomic study substantiates the intensive care unit as a reservoir for carbapenem-resistant in a teaching hospital in China.基因组研究证实，在中国的一家教学医院中，重症监护病房是耐碳青霉烯肠杆菌的储库。
Microb Genom. 2024 Sep;10(9). doi: 10.1099/mgen.0.001299.
4
Transferable AmpCs in : interplay with peptidoglycan recycling, mechanisms of hyperproduction, and virulence implications.可转移 AmpC 在：与肽聚糖回收的相互作用、过度产生的机制以及毒力影响。
Antimicrob Agents Chemother. 2024 May 2;68(5):e0131523. doi: 10.1128/aac.01315-23. Epub 2024 Mar 22.
5
Transcriptome reveals the role of the gene in mediating antibiotic resistance through cell envelope modulation in SCCF01.转录组揭示了该基因在介导SCCF01中通过细胞包膜调节产生抗生素耐药性方面的作用。
Front Microbiol. 2024 Jan 4;14:1295065. doi: 10.3389/fmicb.2023.1295065. eCollection 2023.
6
Nitroxoline resistance is associated with significant fitness loss and diminishes virulence of .硝呋太尔耐药与显著的适应性丧失有关，并降低了 的毒力。
Microbiol Spectr. 2024 Jan 11;12(1):e0307923. doi: 10.1128/spectrum.03079-23. Epub 2023 Dec 8.
7
In vitro Activity of Cefepime/Avibactam Against Carbapenem Resistant and Integrative Metabolomics-Proteomics Approach for Resistance Mechanism: A Single-Center Study.头孢吡肟/阿维巴坦对碳青霉烯耐药菌的体外活性及耐药机制的整合代谢组学-蛋白质组学方法：一项单中心研究
Infect Drug Resist. 2023 Sep 11;16:6061-6077. doi: 10.2147/IDR.S420898. eCollection 2023.
8
Quinolone Resistance of Revealed through Genome and Transcriptome Analyses.通过基因组和转录组分析揭示喹诺酮耐药性。
Int J Mol Sci. 2021 Sep 17;22(18):10036. doi: 10.3390/ijms221810036.
9
Mutations in porin LamB contribute to ceftazidime-avibactam resistance in KPC-producing Klebsiella .产碳青霉烯酶肺炎克雷伯菌中孔蛋白 LamB 的突变导致头孢他啶-阿维巴坦耐药。
Emerg Microbes Infect. 2021 Dec;10(1):2042-2051. doi: 10.1080/22221751.2021.1984182.
10
Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.金属β-内酰胺酶在多药耐药时代：从结构和机制到进化、传播和抑制剂设计。
Chem Rev. 2021 Jul 14;121(13):7957-8094. doi: 10.1021/acs.chemrev.1c00138. Epub 2021 Jun 15.
本文引用的文献
1
Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK.破坏英国产耐厄他培南克雷伯菌和肠杆菌临床分离株中孔蛋白表达的分子机制。
J Antimicrob Chemother. 2009 Apr;63(4):659-67. doi: 10.1093/jac/dkp029. Epub 2009 Feb 20.
2
High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe.肺炎克雷伯菌临床分离株中的高水平碳青霉烯耐药性是由bla(ACT-1)β-内酰胺酶产生、孔蛋白OmpK35/36插入失活以及磷酸盐转运孔蛋白phoe的下调共同导致的。
Antimicrob Agents Chemother. 2006 Oct;50(10):3396-406. doi: 10.1128/AAC.00285-06.
3
Characterization of a large outbreak by CTX-M-1-producing Klebsiella pneumoniae and mechanisms leading to in vivo carbapenem resistance development.产CTX-M-1型肺炎克雷伯菌引起的大规模暴发的特征及体内碳青霉烯类耐药性产生的机制
J Clin Microbiol. 2006 Aug;44(8):2831-7. doi: 10.1128/JCM.00418-06.
4
Role of beta-lactamases and porins in resistance to ertapenem and other beta-lactams in Klebsiella pneumoniae.β-内酰胺酶和孔蛋白在肺炎克雷伯菌对厄他培南及其他β-内酰胺类抗生素耐药中的作用
Antimicrob Agents Chemother. 2004 Aug;48(8):3203-6. doi: 10.1128/AAC.48.8.3203-3206.2004.
5
Role of Klebsiella pneumoniae OmpK35 porin in antimicrobial resistance.肺炎克雷伯菌外膜孔蛋白OmpK35在抗菌耐药性中的作用。
Antimicrob Agents Chemother. 2003 Oct;47(10):3332-5. doi: 10.1128/AAC.47.10.3332-3335.2003.
6
Modification of outer membrane protein profile and evidence suggesting an active drug pump in Enterobacter aerogenes clinical strains.产气肠杆菌临床菌株外膜蛋白谱的改变及提示存在活性药物泵的证据。
Antimicrob Agents Chemother. 2003 May;47(5):1555-9. doi: 10.1128/AAC.47.5.1555-1559.2003.
7
Identification and characterization of a new porin gene of Klebsiella pneumoniae: its role in beta-lactam antibiotic resistance.肺炎克雷伯菌一个新孔蛋白基因的鉴定与特性分析：其在β-内酰胺类抗生素耐药性中的作用
J Bacteriol. 1999 May;181(9):2726-32. doi: 10.1128/JB.181.9.2726-2732.1999.
8
Porin expression in clinical isolates of Klebsiella pneumoniae.肺炎克雷伯菌临床分离株中的孔蛋白表达
Microbiology (Reading). 1999 Mar;145 ( Pt 3):673-679. doi: 10.1099/13500872-145-3-673.
9
Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems.来自临床样本的克隆相关肺炎克雷伯菌分离株的外膜轮廓以及头孢菌素和碳青霉烯类药物的活性
Antimicrob Agents Chemother. 1998 Jul;42(7):1636-40. doi: 10.1128/AAC.42.7.1636.
10
Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.外膜蛋白TolC（mar-sox调控子的一个可能成员）参与维持和提高大肠杆菌K-12对有机溶剂的耐受性。
J Bacteriol. 1998 Feb;180(4):938-44. doi: 10.1128/JB.180.4.938-944.1998.
Zotero 插件