Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Vet Pathol. 2011 Nov;48(6):1190-4. doi: 10.1177/0300985810396516. Epub 2011 Jan 31.
Forty cases of equine penile disease were screened with polymerase chain reaction for the presence of papillomaviral DNA. Cases consisted of 20 squamous cell carcinomas (average age of horse, 23.9 years) and 20 non-squamous cell carcinoma diseases (average age of horse, 13.3 years). All horses but one originated from the Northeastern United States. Breeds were not recorded. As based on MY09/MY11 consensus primers, DNA sequences from equine papillomavirus type 2 were amplified from 9 of 20 horses (45%) with penile squamous cell carcinoma and only 1 of 20 horses (5%) with non-squamous cell carcinoma penile disease. Equine papillomavirus type 2 DNA was the only papillomaviral DNA amplified from any of the 40 horses. Tissues from the 10 horses in which papillomaviral DNA was detected by polymerase chain reaction were also screened with in situ hybridization and immunohistochemistry. The presence of papillomavirus was demonstrated in a subset of these by in situ hybridization (6 of 10) and immunohistochemistry (1 of 10). This report describes a possible association between equine penile squamous cell carcinomas and equine papillomavirus type 2. This study is also the first report of equine papillomavirus type 2 infection in North American horses.
40 例马阴茎疾病病例通过聚合酶链反应（PCR）筛选是否存在乳头瘤病毒 DNA。这些病例包括 20 例鳞状细胞癌（马的平均年龄为 23.9 岁）和 20 例非鳞状细胞癌疾病（马的平均年龄为 13.3 岁）。除了一匹马外，所有的马都来自美国东北部。未记录品种。根据 MY09/MY11 共识引物，从 20 匹患有阴茎鳞状细胞癌的马中（45%）和 20 匹非鳞状细胞癌阴茎疾病的马中（5%）扩增了 9 匹（45%）马的 2 型马乳头瘤病毒 DNA。从这 40 匹马中的任何一匹马中扩增的唯一乳头瘤病毒 DNA 都是 2 型马乳头瘤病毒 DNA。用聚合酶链反应检测到乳头瘤病毒 DNA 的 10 匹马的组织也用原位杂交和免疫组织化学进行了筛选。原位杂交（6/10）和免疫组织化学（1/10）在这些组织中证实了乳头瘤病毒的存在。本报告描述了马阴茎鳞状细胞癌与 2 型马乳头瘤病毒之间可能存在的关联。本研究也是北美马感染 2 型马乳头瘤病毒的首次报告。
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