Alloway Elizabeth, Linder Keith, May Susan, Rose Trevor, DeLay Josepha, Bender Susan, Tucker Alison, Luff Jennifer
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Animal Health Laboratory, University of Guelph, Guelph, Ontario, Canada.
Vet Pathol. 2020 May;57(3):427-431. doi: 10.1177/0300985820908797. Epub 2020 Mar 17.
Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As papillomavirus-2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.
鳞状细胞癌（SCC）是马胃最常见的肿瘤。然而，恶性转化的潜在机制尚不清楚。由于乳头瘤病毒2型（EcPV-2）可能是某些生殖器SCC的病因，我们推测EcPV-2与一部分马胃SCC有关。为此，我们对11例胃SCC以及15例无SCC的对照马的胃样本进行了EcPV-2 E6/E7癌基因的聚合酶链反应（PCR）和原位杂交（ISH）。11例胃SCC中有7例（64%）EcPV-2 PCR呈阳性；非SCC胃样本均为阴性。通过ISH在5/11（45%）的胃SCC肿瘤细胞中检测到EcPV-2 E6/E7核酸的强烈杂交信号，包括远处转移灶。在任何非SCC胃病例中均未检测到杂交信号。本研究为EcPV-2感染与一部分马胃SCC之间的潜在关联提供了支持。
