糖蛋白在猫科动物睾丸精子、附睾精子和射出精子上的分布。

Distribution of glycoproteins on feline testicular sperm, epididymal sperm and ejaculated sperm.

作者信息

Toyonaga Mari, Morita Miki, Hori Tatsuya, Tsutsui Toshihiko

机构信息

Department of Reproduction, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan.

出版信息

J Vet Med Sci. 2011 Jun;73(6):827-9. doi: 10.1292/jvms.10-0400. Epub 2011 Jan 26.

DOI:10.1292/jvms.10-0400

PMID:21282936

Abstract

Glycoproteins (GPs) are known to be involved in the phenomenon of sperm maturation and capacitation. In the present study, we investigated the attachment of GPs on sperm cell membrane during the process of feline sperm maturation from testicular sperm to ejaculated sperm by using 8 FITC-labeled lectins. The results showed that 3 types of GPs were presented on testicular sperm and 7 on caput epididymal sperm. Corpus and cauda epididymal sperm and ejaculated sperm had GPs detected by 8 FITC-labeled lectins used in the present study. This study demonstrates the part of the characteristic of GPs that are present on the feline sperm cell membrane during the process of sperm maturation.

摘要

已知糖蛋白（GPs）参与精子成熟和获能现象。在本研究中，我们通过使用8种异硫氰酸荧光素（FITC）标记的凝集素，研究了猫精子从睾丸精子到射出精子的成熟过程中糖蛋白在精子细胞膜上的附着情况。结果显示，睾丸精子上存在3种类型的糖蛋白，附睾头精子上存在7种。本研究中使用的8种FITC标记凝集素均检测到附睾体和附睾尾精子以及射出精子上有糖蛋白。这项研究揭示了精子成熟过程中猫精子细胞膜上糖蛋白的部分特征。