犬睾丸、附睾、输精管及射出精子表面凝集素结合位点的变化。

Changes in the lectin binding sites on the testicular, epididymal, vas, and ejaculated spermatozoon surface of dog.

作者信息

Bains H K, Pabst M A, Bawa S R

机构信息

Department of Biophysics, Panjab University, Chandigarh, India.

出版信息

Andrologia. 1993 Jan-Feb;25(1):19-24. doi: 10.1111/j.1439-0272.1993.tb02676.x.

DOI:10.1111/j.1439-0272.1993.tb02676.x

PMID:8427417

Abstract

Changes in the localization of sperm surface glycocomponents of testicular, epididymal, vas deferens, and ejaculated spermatozoa of dog (Canis domesticus) were studied employing fluorescein isothiocyanate conjugated lectins viz., Concanavalin A (ConA), Triticum vulgaris (WGA), Maclura pomifera (MPA), and Arachis hypogaea (PNA) agglutinins. The plasma membrane clothing the acrosome of the testicular, epididymal, and vas deferens spermatozoa shows reactivity with all the lectins used. However, in the ejaculated spermatozoa, the entire sperm surface shows reactive sites for ConA, WGA, and PNA. Variation in the labelling of the cytoplasmic droplet in different stages of spermatozoon transit in the epididymis has been discussed.

摘要

利用异硫氰酸荧光素偶联凝集素，即刀豆球蛋白A（ConA）、普通小麦（WGA）、桑科柘属植物（MPA）和落花生（PNA）凝集素，研究了犬（家犬）睾丸、附睾、输精管和射出精子的精子表面糖组分定位变化。覆盖睾丸、附睾和输精管精子顶体的质膜与所有使用的凝集素均显示出反应性。然而，在射出的精子中，整个精子表面显示出对ConA、WGA和PNA的反应位点。文中讨论了附睾中精子运输不同阶段细胞质滴标记的变化。