Department of Ophthalmology, Ophthalmic Pathology Laboratory, Cullen Eye Institute, Baylor College of Medicine, Houston, TX, USA.
Cornea. 2011 Jul;30(7):807-14. doi: 10.1097/ICO.0b013e318201440c.
The purpose of this study was to evaluate clinical cases of cat scratch disease (CSD) and bacillary angiomatosis involving the conjunctiva by special stains and transmission electron microscopy (TEM) and to compare these findings with the results from species-specific polymerase chain reaction (PCR) analysis of the same specimens.
Six potential cases of CSD and 2 possible cases of bacillary angiomatosis of the conjunctiva were analyzed by light microscopy, the Warthin-Starry technique, TEM, and PCR. DNA isolated from cultured Bartonella henselae, B. bacilliformis, B. quintana, and B. elizabethae were used as control templates for establishment of the PCR sensitivity and specificity. Cultured DNA was also used as appropriate positive controls during analysis of the clinical specimens.
The histological studies, electron microscopy, and the PCR analysis confirmed the identification of the bacilli within the involved tissues. Furthermore, molecular diagnosis by PCR allowed for speciation of the infecting Bartonella organisms in 6 of the 8 cases and correlated with the histological findings.
The PCR-based identification of Bartonella correlated well with the results of light microscopy and TEM and provided a simple and rapid method of diagnosis to the species level. The molecular analysis may prove to be beneficial in enhancing the current diagnostic techniques for CSD and bacillary angiomatosis.
本研究旨在通过特殊染色和透射电子显微镜（TEM）评估结膜部位的猫抓病（CSD）和杆菌性血管瘤病的临床病例，并将这些发现与同一标本的种特异性聚合酶链反应（PCR）分析结果进行比较。
通过光镜、华氏染色技术、TEM 和 PCR 分析了 6 例疑似 CSD 和 2 例疑似结膜杆菌性血管瘤病。Bartonella henselae、B. bacilliformis、B. quintana 和 B. elizabethae 的培养物 DNA 被用作建立 PCR 灵敏度和特异性的对照模板。在分析临床标本时，还使用培养的 DNA 作为适当的阳性对照。
组织学研究、电子显微镜和 PCR 分析证实了在受累组织中存在杆菌。此外，PCR 分子诊断能够对 8 例中的 6 例感染的巴尔通体进行种属鉴定，并与组织学发现相关。
基于 PCR 的巴尔通体鉴定与光镜和 TEM 的结果非常吻合，并提供了一种简单、快速的种属水平诊断方法。分子分析可能有助于增强当前 CSD 和杆菌性血管瘤病的诊断技术。
