Beigi Boroujeni Mandana, Salehnia Mojdeh, Khalatbary Ali Reza, Pourbeiranvand Shahram, Beigi Boroujeni Nasim, Ebrahimi Saedeh
Dept. of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
Dept. of Anatomical Sciences, Tarbiat Modares University, School of Medicine, Tehran, Iran.
Iran Biomed J. 2010 Oct;14(4):171-7.
Apoptosis is a process that plays an important role during early stage of implantation. The aim of this study was to investigate the incidence of apoptosis in mice endometrium after ovarian stimulation at implantation period.
NMRI female mice were divided into two groups: 1) control group, which were rendered pseudopregnant by vaginal stimulation and 2) experimental group, which were stimulated using an intrapritoneal injection of 10 IU hMG followed by another injection of 10 IU hCG after 48 h. In the evening of the second injection, the mice were rendered pseudopregnant the same as control group. Samples were obtained from 1/3 middle part of uterine horns during implantation period. Apoptosis was assessed in two groups at implantation period using light and electron microscopic studies, TUNEL staining and semiquantitative RT-PCR.
Our morphological and ultrastructural results showed apoptosis in both groups, while TUNEL analysis showed that the percentage of TUNEL-positive cells was higher in stimulated group than in the control group (P≤0.05). The expression of P53, Fas and FasL mRNA was similar in two groups but Bax and Bcl2 were much higher in control group than in the stimulated group (P≤0.05). The ratio of Bax/Bcl2 expression was much higher in stimulated group than in the control group (P≤0.05).
The ovarian stimulation could change the expression of some apoptosis-related genes and enhance the incidence of endometrial apoptosis at implantation period; thus, it could affect on the implantation rate and endometrial receptivity.
细胞凋亡是在着床早期发挥重要作用的一个过程。本研究的目的是调查在着床期卵巢刺激后小鼠子宫内膜中细胞凋亡的发生率。
将NMRI雌性小鼠分为两组：1）对照组，通过阴道刺激使其假孕；2）实验组，腹腔注射10 IU人绝经期促性腺激素（hMG）进行刺激，48小时后再注射10 IU人绒毛膜促性腺激素（hCG）。在第二次注射的当晚，使小鼠与对照组一样假孕。在着床期从子宫角中部的1/3处获取样本。使用光镜和电镜研究、TUNEL染色和半定量逆转录聚合酶链反应（RT-PCR）在着床期对两组进行细胞凋亡评估。
我们的形态学和超微结构结果显示两组均有细胞凋亡，而TUNEL分析显示刺激组中TUNEL阳性细胞的百分比高于对照组（P≤0.05）。两组中P53、Fas和FasL mRNA的表达相似，但对照组中Bax和Bcl2的表达远高于刺激组（P≤0.05）。刺激组中Bax/Bcl2表达的比率远高于对照组（P≤0.05）。
卵巢刺激可改变一些凋亡相关基因的表达，并增加着床期子宫内膜细胞凋亡的发生率；因此，它可能影响着床率和子宫内膜容受性。
