Beigi Boroujeni Mandana, Beigi Boroujeni Nasim, Salehnia Mojdeh, Marandi Elahe, Beigi Boroujeni Masoud
Dept. of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Dept. of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Iran Biomed J. 2012;16(1):33-7. doi: 10.6091/ibj.1033.2012.
To achieve multiple oocytes for in vitro fertilization, ovulation induction is induced by gonadotropins; however, it has several effects on oocytes and embryo quality and endometrium receptivity. The aim of this study was to assess ultrastructural changes of corpus luteum after ovarian induction using human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG) during luteal phase at implantation period.
Female NMRI mice (6-8 weeks) were divided into control and stimulated groups. In the control group, the mice were rendered pseudopregnant and in the ovarian induction group, the mice were rendered pseudopregnant after the ovarian induction. The samples were obtained from the ovary in each group at the same time during luteal phase at implantation period. Ultrastructural changes were assessed using electron microscopy study.
Our results displayed some identifiable changes in ultrastructure of corpus luteum in ovarian induction group. These changes included enhancement of the apoptosis and intercellular space, whereas the angiogenesis was decreased. The findings indicated a decline in organelle density in the cytoplasm of ovarian induction, such as mitochondria, endoplasmic reticulum and polyribosome. Furthermore, chromatin condensation of nuclei was observed in some cells.
The ovarian induction using HMG and HCG resulted in some ultrastructural changes on the corpus luteum at implantation period, which could affect on the pregnancy rate.
为了获得多个用于体外受精的卵母细胞，使用促性腺激素诱导排卵；然而，它对卵母细胞、胚胎质量和子宫内膜容受性有多种影响。本研究的目的是评估在植入期黄体期使用人绝经期促性腺激素（HMG）和人绒毛膜促性腺激素（HCG）进行卵巢诱导后黄体的超微结构变化。
将6-8周龄的雌性NMRI小鼠分为对照组和刺激组。对照组小鼠诱导假孕，卵巢诱导组小鼠在卵巢诱导后诱导假孕。在植入期黄体期的同一时间从每组的卵巢中获取样本。使用电子显微镜研究评估超微结构变化。
我们的结果显示卵巢诱导组黄体的超微结构有一些可识别的变化。这些变化包括细胞凋亡增加和细胞间隙增大，而血管生成减少。研究结果表明卵巢诱导组细胞质中的细胞器密度下降，如线粒体、内质网和多核糖体。此外，在一些细胞中观察到细胞核染色质浓缩。
在植入期使用HMG和HCG进行卵巢诱导导致黄体出现一些超微结构变化，这可能会影响妊娠率。
