Bone Regeneration Laboratory, Rizzoli Orthopaedic Institute, 40136 Bologna, Italy.
J Orthop Res. 2011 Jun;29(6):961-8. doi: 10.1002/jor.21333. Epub 2011 Jan 31.
The objective of this study was to evaluate whether mesenchymal stem cells (MSC) and platelet lysate (PL) seeded in a fibrin or collagen scaffold could improve the new bone (NB) formation around an uncemented hip prosthesis stem in a sheep model. In vitro expanded MSC were suspended in PL and either mixed with collagen or fibrin gel as delivery vehicle. The cell-gel composites were inserted inside the femoral canal, then the prosthesis was press-fit inserted inside the femur. Identical procedures were performed in a control group, but only the prosthesis was implanted. Histomorphometrical analysis performed 4 months after surgery indicated that the newly formed bone inside the medullary canal, between the inner cortex and the prosthetic stem, was significantly higher in the MSC-PL-collagen group (mean 18.7 ± 4.5%) and in the MSC-PL-fibrin group (mean 18.8 ± 15.2%) when compared to the control group (mean 4.6 ± 2.0%). There was a significantly higher bone-prosthesis contact in the MSC-PL-collagen group (mean 2.7 ± 2.6%) and in the MSC-PL-fibrin group (mean 2.3 ± 3.1%) compared to the control group (mean 0.2 ± 0.1%). The results indicate that MSC and PL in a fibrin or collagen scaffold can promote NB formation around an uncemented hip prosthesis stem.
本研究旨在评估骨髓间充质干细胞（MSC）和血小板裂解液（PL）接种于纤维蛋白或胶原支架中是否能改善非骨水泥髋关节假体柄周围的新骨（NB）形成。体外扩增的 MSC 悬浮于 PL 中，并与胶原或纤维蛋白凝胶混合作为输送载体。将细胞-凝胶复合物插入股骨髓腔内部，然后将假体压入股骨。在对照组中进行了相同的程序，但仅植入了假体。术后 4 个月进行的组织形态计量学分析表明，在 MSC-PL-胶原组（平均 18.7±4.5%）和 MSC-PL-纤维蛋白组（平均 18.8±15.2%）中，骨髓腔内、内皮层和假体柄之间形成的新骨明显高于对照组（平均 4.6±2.0%）。MSC-PL-胶原组（平均 2.7±2.6%）和 MSC-PL-纤维蛋白组（平均 2.3±3.1%）的骨-假体接触明显高于对照组（平均 0.2±0.1%）。结果表明，纤维蛋白或胶原支架中的 MSC 和 PL 可促进非骨水泥髋关节假体柄周围 NB 的形成。
