Department of BioMedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Tissue Eng Part C Methods. 2011 May;17(5):607-17. doi: 10.1089/ten.TEC.2010.0556. Epub 2011 Mar 4.
In autologous heart valve tissue engineering, there is an ongoing search for alternatives of fetal bovine serum (FBS). Human platelet-lysate (PL) might be a promising substitute. In the present article, we aimed to examine the tissue formation, functionality, and mechanical properties of engineered three-dimensional tissue constructs cultured in PL as a substitute for FBS. Our results show that tissue constructs that were cultured in PL and FBS produce similar amounts of collagen, glycosoaminoglycans, and collagen crosslinks, and that the cellular phenotype remains unchanged. Nevertheless, mechanical testing showed that the ultimate tensile strength in PL constructs was on average approximately three times lower as compared to FBS (0.25 vs. 0.74 MPa, respectively, p<0.01), and also the elastic modulus was almost three times lower (1.33 MPa of PL constructs vs. 3.94 MPa of FBS constructs, p<0.01). Additional tests indicated that this difference might be explained by different collagen fiber architecture possibly due to increased production of matrix-degrading proteases by cells cultured in PL. In summary, our results indicate that PL is not preferred for the culture of strong heart valve tissue constructs.
在自体心脏瓣膜组织工程中，人们一直在寻找胎牛血清（FBS）的替代品。人血小板裂解液（PL）可能是一种很有前途的替代品。在本文中，我们旨在研究在 PL 中培养作为 FBS 替代品的工程化三维组织构建体的组织形成、功能和机械性能。我们的结果表明，在 PL 和 FBS 中培养的组织构建体产生相似量的胶原、糖胺聚糖和胶原交联，并且细胞表型保持不变。然而，力学测试表明，PL 构建体的最大拉伸强度平均比 FBS 低约三倍（分别为 0.25 和 0.74 MPa，p<0.01），弹性模量也几乎低三倍（PL 构建体为 1.33 MPa，FBS 构建体为 3.94 MPa，p<0.01）。进一步的测试表明，这种差异可能是由于 PL 培养的细胞可能产生更多的基质降解蛋白酶，导致胶原纤维结构不同。总之，我们的结果表明，PL 不适合培养强心脏瓣膜组织构建体。
