Department of Biochemistry, Basic Medical Science Block, Panjab University, Chandigarh, India.
Free Radic Res. 2011 May;45(5):559-67. doi: 10.3109/10715762.2011.555765. Epub 2011 Feb 2.
Increased oxidative stress and lymphocyte apoptosis are a hallmark of the autoimmune disease systemic lupus erythematosus (SLE). However, the association between oxidative stress and T lymphocytes apoptosis has still to be elucidated in SLE. In order to appraise the interaction between oxidative stress and T lymphocyte apoptosis with the severity of disease, oxidative stress profile and T lymphocytes apoptosis were studied. Increased levels of ROS, MDA and CD4(+) lymphocyte apoptosis were positively associated with disease activity while decreased levels of GSH and percentage expression of CD4(+) lymphocyte were negatively associated with disease activity. The decrease in intracellular levels of GSH was negatively associated with T lymphocyte, CD4(+) lymphocyte, CD8(+) lymphocyte apoptosis and intracellular caspase-3 expression. The present study suggests that increased T lymphocyte sub-sets apoptosis may be mediated by decreased intracellular glutathione concentration and severity of disease might be enhanced together by over-production of ROS in SLE.
氧化应激和淋巴细胞凋亡增加是红斑狼疮（SLE）等自身免疫性疾病的一个标志。然而，SLE 中氧化应激与 T 淋巴细胞凋亡之间的关系仍有待阐明。为了评估氧化应激与 T 淋巴细胞凋亡与疾病严重程度之间的相互作用，研究了氧化应激谱和 T 淋巴细胞凋亡。ROS、MDA 和 CD4+淋巴细胞凋亡水平升高与疾病活动度呈正相关，而 GSH 水平和 CD4+淋巴细胞百分率表达降低与疾病活动度呈负相关。细胞内 GSH 水平的降低与 T 淋巴细胞、CD4+淋巴细胞、CD8+淋巴细胞凋亡和细胞内 caspase-3 表达呈负相关。本研究表明，T 淋巴细胞亚群凋亡的增加可能是由细胞内谷胱甘肽浓度降低介导的，ROS 的过度产生可能与疾病的严重程度共同增强。
