使用适体和金纳米粒子通过逐步稀释对血清中的凝血酶进行定性和定量分析。
Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution.
机构信息
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
出版信息
Anal Biochem. 2011 Jun 1;413(1):75-7. doi: 10.1016/j.ab.2011.01.032. Epub 2011 Feb 1.
It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles.
在使用适体和金纳米粒子进行生物测定以直接检测复杂生物样品（如血清）中的疾病相关蛋白和其他物质方面，这一直是一个挑战。在这里，我们提出了一种渐进稀释（PD）方法，无需对样品进行预处理，即可实现对血清中蛋白质的定性和定量分析。在检测限以上，PD 具有无限的动态范围。我们通过使用金纳米粒子的荧光猝灭来检测胎牛血清中的凝血酶，证明了 PD 策略的可行性。
Zotero 插件