Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, P. R. China.
Small. 2010 Jan;6(2):201-4. doi: 10.1002/smll.200901012.
The design of a novel multicolor fluorescent gold nanoprobe for homogeneous detection of small-molecule targets is reported, which combines the specific binding abilities of aptamers with the ultrahigh quenching ability of gold nanoparticles (AuNPs). Dye-tagged aptamers and their complementary sequence with thiol labels are co-assembled at the surface of AuNPs. As a proof of concept, it is demonstrated that such a multicolor fluorescent gold nanoprobe can simultaneously detect adenosine, potassium ion, and cocaine with high selectivity. This potentially generic strategy is shown to be promising for rapid screening of small molecular targets.
报道了一种新型多色荧光金纳米探针的设计，用于均相检测小分子靶标，该探针结合了适体的特异性结合能力和金纳米粒子（AuNPs）的超高猝灭能力。带有染料标记的适体及其带有巯基标记的互补序列在 AuNPs 表面共组装。作为概念验证，证明了这种多色荧光金纳米探针可以同时高选择性地检测腺苷、钾离子和可卡因。这种潜在的通用策略有望用于快速筛选小分子靶标。
