Oreland L, Jossan S S, Hartvig P, Aquilonius S M, Långström B
Department of Medical Pharmacology, University Hospital, Uppsala, Sweden.
J Neural Transm Suppl. 1990;32:55-9. doi: 10.1007/978-3-7091-9113-2_6.
Positron emission tomography (PET) was applied to investigate the rate of turnover of pig brain MAO-B. For this purpose 11C-L-deprenyl was used as an irreversible ligand, which bind stoichiometrically to the enzyme. A tracer dose of 11C-L-deprenyl was injected and PET scans performed to obtain baseline deprenyl binding. A high dose of unlabelled deprenyl was then administered to inhibit the enzyme and tracer doses of 11C-L-deprenyl, with subsequent PET analyses, were given at 0, 2, 7, 21 and 42 days. The half-life for the turnover rate calculated was found to be 6.5 days.
正电子发射断层扫描（PET）被用于研究猪脑单胺氧化酶B（MAO-B）的周转率。为此，使用11C-L-司来吉兰作为不可逆配体，它能与该酶按化学计量结合。注射示踪剂量的11C-L-司来吉兰并进行PET扫描以获得司来吉兰结合的基线数据。然后给予高剂量的未标记司来吉兰以抑制该酶，并在0、2、7、21和42天分别给予示踪剂量的11C-L-司来吉兰并随后进行PET分析。计算得出的周转率半衰期为6.5天。
