Department of Obstetrics and Gynecology and Reproductive Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USA.
Reprod Sci. 2011 Aug;18(8):723-9. doi: 10.1177/1933719110396700. Epub 2011 Feb 1.
This study was conducted to determine whether gestational changes in maternal uterine artery reactivity are primarily driven by local vs. systemic factors. Rats underwent surgical ligation of one oviduct, thereby restricting implantation and pregnancy to one uterine horn while maintaining a gestational endocrine milieu. Uterine arcuate arteries were isolated and cannulated to evaluate reactivity. Vessels from the implanted horn were significantly more sensitive to phenylephrine and less sensitive to sodium nitroprusside than those from the non-implanted horn; endothelial basal calcium levels were only increased in the implanted horn. Conversely, there were no differences in sensitivity to acetylcholine, or its effects on endothelial cell calcium, although efficacy was greater in vessels from the implanted vs. non-implanted horn. These findings demonstrate that local factors are predominant in inducing changes in vascular smooth muscle function, while endothelial adaptations result from an interplay between local and systemic factors, with distinct effects attributable to each.
本研究旨在确定母体子宫动脉反应性的妊娠变化主要是由局部因素还是全身因素驱动。大鼠接受单侧输卵管结扎手术，从而将着床和妊娠限制在一个子宫角，同时保持妊娠内分泌环境。分离并插管子宫弓形动脉以评估反应性。与非着床角的血管相比，着床角的血管对苯肾上腺素的敏感性更高，对硝普钠的敏感性更低；着床角的血管内皮基础钙水平仅升高。相反，乙酰胆碱的敏感性及其对内皮细胞钙的影响没有差异，尽管植入角的血管比非植入角的血管的效能更大。这些发现表明，局部因素在诱导血管平滑肌功能变化方面占主导地位，而内皮适应则是局部和全身因素相互作用的结果，每个因素都有其独特的影响。
