Freitas T V, Fortes-Dias C L, Diniz C R
Fundação Ezequiel Dias, Belo Horizonte (MG), Brazil.
Toxicon. 1990;28(12):1491-6. doi: 10.1016/0041-0101(90)90162-z.
Mice and rabbits were immunized against crotoxin (the neurotoxic component isolated from Crotalus durissus terrificus venom) using small amounts of antigen in a water-in-oil emulsion. Following boosting (three times at 21-day intervals) a high titre of antibodies against crotoxin was obtained. Crotoxin immunoglobulin G antibody recognizes whole venom antigen at a level comparable with that of crotoxin antigen, using the ELISA method for antibody detection. The antibodies generated by crotoxin were capable of providing 100% protection against challenge with 11 and 50 i.p. LD50 doses of whole venom in mice. When 100 i.p. LD50 doses of whole venom were injected survival was 77.8%.
使用少量抗原在油包水乳液中对小鼠和兔子进行抗响尾蛇毒素（从剧毒矛头蝮蛇毒中分离出的神经毒性成分）免疫。在加强免疫（每隔21天进行三次）后，获得了高滴度的抗响尾蛇毒素抗体。使用ELISA方法检测抗体时，响尾蛇毒素免疫球蛋白G抗体识别全毒液抗原的水平与响尾蛇毒素抗原相当。响尾蛇毒素产生的抗体能够为小鼠提供100%的保护，使其免受11和50个腹腔注射半数致死剂量全毒液的攻击。当注射100个腹腔注射半数致死剂量的全毒液时，存活率为77.8%。
