Mateva V, Tekerlekov P
Vet Med Nauki. 1978;15(2):101-7.
Experiments were carried out to use the agar gel diffusion test (AGDT) in the study of bovine leukosis. An antigen was obtained from FLK-24 cells with a latent leukosis virus infection, which proved specific and suitable for AGDT. A total of 134 serum samples were examined applyig AGDT, for the presence of specific antibodies, originating from cattle of herds in which leukosis had been hematologically demonstrated. Investigated were also 56 sera of animals presenting an unknown clinical picture. Positive reaction was established in 70 per cent of the sera taken from herds with hematologically demonstrated leukosis. The specificity of AGDT was confirmed through establishing specific antibodies also in sera of lambs experimentally infected with a virus from a FBL-J 5 cell line with a latent infection. It is admitted that AGDT is a specific, sensitive, and readily applicable method, which can successfully be used in the diagnostics of leukosis in this country.
开展了多项实验，以利用琼脂凝胶扩散试验（AGDT）研究牛白血病。从感染潜伏性白血病病毒的FLK - 24细胞中获得了一种抗原，该抗原经证明具有特异性且适用于AGDT。总共对134份血清样本进行了AGDT检测，以确定来自血液学诊断患有白血病的牛群的牛血清中是否存在特异性抗体。还对56份临床表现不明的动物血清进行了检测。在血液学诊断患有白血病的牛群所采集的血清中，70%检测出阳性反应。通过在实验感染来自具有潜伏感染的FBL - J 5细胞系病毒的羔羊血清中也检测到特异性抗体，证实了AGDT的特异性。公认AGDT是一种特异性强、灵敏度高且易于应用的方法，可成功用于该国白血病的诊断。
