Wawrzkiewicz J, Dziedzic B, Kozioł T
Department of Microbiology, Institute of Infectious and Invasive Diseases, Veterinary Faculty, Agriculture Academy, Lublin, Poland.
Acta Virol. 1989 Mar;33(2):143-50.
Sensitivity and specificity of a modified agar gel immuno-diffusion (AGID) test for the diagnosis of enzootic bovine leukosis was compared in 609 sera using the standard method, the modified test, and ELISA. The modifications concerned the composition of agar gel (1.8% Bacto-agar Difco), addition of polyethylene glycol (PEG 6000, 4%) and enlarging the diameters of the wells from 6 to 10 mm for sera. The change in well diameters and the addition of PEG 6000 increased the sensitivity of the test by about 60-100%, favourably influenced the proper interpretation of the results, especially with weak positive sera. ELISA proved to be more sensitive either than the modified (by 4.1%) or standard AGID (by 5.25%) tests. The modified AGID test may find wide application in laboratory practice, especially when a more sensitive technique is not available.
采用标准方法、改良试验和酶联免疫吸附测定（ELISA），对609份血清进行检测，比较改良琼脂凝胶免疫扩散（AGID）试验诊断地方流行性牛白血病的敏感性和特异性。改良之处包括琼脂凝胶的成分（1.8% 迪夫科细菌琼脂）、添加聚乙二醇（PEG 6000，4%）以及将血清孔的直径从6毫米扩大到10毫米。孔直径的改变和PEG 6000的添加使试验的敏感性提高了约60 - 100%，对结果的正确判读产生了有利影响，尤其是对于弱阳性血清。ELISA被证明比改良AGID试验（高4.1%）或标准AGID试验（高5.25%）更敏感。改良AGID试验在实验室实践中可能会有广泛应用，特别是在没有更敏感技术可用时。
